Brian T Crouch

Research Interests

Developing novel, low cost technologies for cancer diagnostics and therapeutics with applications in resource limited settings. Particular focus on innovative strategies to induce a robust anti-tumor immune response following ablative therapy.

Bio

Dr. Crouch is an Assistant Research Professor in the Department of Biomedical Engineering and the Assistant Director of Research for the Center for Global Women's Health Technologies (GWHT). Dr. Crouch’s research interests intersect oncology, translational research, and global health, with a particular focus on diagnostics and therapeutics in low-resource settings. He is an expert in translating diagnostic technologies from the bench to bedside using in vitro cell culture and cell-based assays, small animal models, fluorescence microscopy, image processing, machine learning, and clinical trial design.

Education

• Ph.D. Duke University, 2018

Positions

• Assistant Research Professor in the Department of Biomedical Engineering

Courses Taught

• GLHLTH 796T: Bass Connections Global Health Research Team
• GLHLTH 795T: Bass Connections Global Health Research Team
• GLHLTH 396T: Bass Connections Global Health Research Team
• GLHLTH 395T: Bass Connections Global Health Research Team
• EGR 491: Projects in Engineering
• EGR 393: Research Projects in Engineering
• BME 494: Projects in Biomedical Engineering (GE)
• BME 493: Projects in Biomedical Engineering (GE)

Publications

• Adhikari G, Sarojasamhita VP, Powell VR, Farooqui A, Budzinski M, Garvey DT, et al. Impact of Injection-Based Delivery Parameters on Local Distribution Volume of Ethyl-Cellulose Ethanol Gel in Tissue and Tissue Mimicking Phantoms. IEEE transactions on bio-medical engineering. 2024 May;71(5):1488–98.
• Chelales E, von Windheim K, Banipal AS, Siebeneck E, Benham C, Nief CA, et al. Determining the Relationship between Delivery Parameters and Ablation Distribution for Novel Gel Ethanol Percutaneous Therapy in Ex Vivo Swine Liver. Polymers (Basel). 2024 Apr 5;16(7).
• Sunassee ED, Deutsch RJ, D’Agostino VW, Castellano-Escuder P, Siebeneck EA, Ilkayeva O, et al. Optical imaging reveals chemotherapy-induced metabolic reprogramming of residual disease and recurrence. Sci Adv. 2024 Apr 5;10(14):eadj7540.
• Quang TT, Yang J, Kaluzienski ML, Parrish A, Farooqui A, Katz D, et al. In Vivo Evaluation of Safety and Efficacy of Ethyl Cellulose-Ethanol Tissue Ablation in a Swine Cervix Model. Bioengineering (Basel, Switzerland). 2023 Oct;10(11):1246.
• Madonna MC, Fox DB, Crouch BT, Lee J, Zhu C, Martinez AF, et al. Data from Optical Imaging of Glucose Uptake and Mitochondrial Membrane Potential to Characterize Her2 Breast Tumor Metabolic Phenotypes. 2023.
• Madonna MC, Fox DB, Crouch BT, Lee J, Zhu C, Martinez AF, et al. Data from Optical Imaging of Glucose Uptake and Mitochondrial Membrane Potential to Characterize Her2 Breast Tumor Metabolic Phenotypes. 2023.
• Madonna MC, Fox DB, Crouch BT, Lee J, Zhu C, Martinez AF, et al. Supplementary figures and captions 1-5 from Optical Imaging of Glucose Uptake and Mitochondrial Membrane Potential to Characterize Her2 Breast Tumor Metabolic Phenotypes. 2023.
• Madonna MC, Fox DB, Crouch BT, Lee J, Zhu C, Martinez AF, et al. Supplementary figures and captions 1-5 from Optical Imaging of Glucose Uptake and Mitochondrial Membrane Potential to Characterize Her2 Breast Tumor Metabolic Phenotypes. 2023.
• Wang R, Alvarez DA, Crouch BT, Pilani A, Lam C, Zhu C, et al. Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis. Biomed Opt Express. 2021 Apr 1;12(4):2299–311.
• Nief C, Morhard R, Chelales E, Adrianzen Alvarez D, Bourla Bs I, Lam CT, et al. Polymer-assisted intratumoral delivery of ethanol: Preclinical investigation of safety and efficacy in a murine breast cancer model. PLoS One. 2021;16(1):e0234535.
• Madonna M, Duer J, Lee J, Williams J, Avsaroglu B, Zhu C, et al. In vivo optical metabolic imaging of long-chain fatty acid uptake in orthotopic models of triple negative breast cancer. 2020;
• Nief C, Morhard R, Chelales E, Alvarez DA, Bourla I, Lam C, et al. Polymer-assisted intratumoral delivery of ethanol: Preclinical investigation of safety and efficacy in a murine breast cancer model. 2020;
• Madonna MC, Fox DB, Crouch BT, Lee J, Zhu C, Martinez AF, et al. Optical Imaging of Glucose Uptake and Mitochondrial Membrane Potential to Characterize Her2 Breast Tumor Metabolic Phenotypes. Mol Cancer Res. 2019 Jul;17(7):1545–55.
• Zhu C, Li M, Vincent T, Martin HL, Crouch BT, Martinez AF, et al. Simultaneous in vivo optical quantification of key metabolic and vascular endpoints reveals tumor metabolic diversity in murine breast tumor models. J Biophotonics. 2019 Apr;12(4):e201800372.
• Crouch BT, Gallagher J, Wang R, Duer J, Hall A, Soo MS, et al. Exploiting heat shock protein expression to develop a non-invasive diagnostic tool for breast cancer. Sci Rep. 2019 Mar 5;9(1):3461.
• Zhu C, Martin HL, Crouch BT, Martinez AF, Li M, Palmer GM, et al. Near-simultaneous quantification of glucose uptake, mitochondrial membrane potential, and vascular parameters in murine flank tumors using quantitative diffuse reflectance and fluorescence spectroscopy. Biomed Opt Express. 2018 Jul 1;9(7):3399–412.
• Martinez AF, McCachren SS, Lee M, Murphy HA, Zhu C, Crouch BT, et al. Metaboloptics: Visualization of the tumor functional landscape via metabolic and vascular imaging. Sci Rep. 2018 Mar 8;8(1):4171.
• Crouch BT, Duer J, Wang R, Gallagher J, Hall A, Soo MS, et al. Optimizing fluorescently-tethered Hsp90 inhibitor dose for maximal specific uptake by breast tumors. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2018.
• Crouch B, Murphy H, Belonwu S, Martinez A, Gallagher J, Hall A, et al. Leveraging ectopic Hsp90 expression to assay the presence of tumor cells and aggressive tumor phenotypes in breast specimens. Sci Rep. 2017 Dec 13;7(1):17487.
• Zhu C, Martinez AF, Martin HL, Li M, Crouch BT, Carlson DA, et al. Near-simultaneous intravital microscopy of glucose uptake and mitochondrial membrane potential, key endpoints that reflect major metabolic axes in cancer. Sci Rep. 2017 Oct 23;7(1):13772.
• Zhu C, Martinez AF, Li M, Martin HL, Crouch B, Hu F, et al. Imaging of 2-NBDG and TMRE reveals glucose uptake and mitochondrial membrane potential in dorsal window chamber models. In: Optics InfoBase Conference Papers. 2017.