Ashutosh Chilkoti

Biomedical Engineering

Acting Chair of Biomedical Engineering, Alan L. Kaganov Distinguished Professor of BME

Ashutosh Chilkoti Profile Photo

Research Interests

Focused on biomolecular materials and biointerface science and emphasizes the development of applications that span the range from bioseparations, biosensors, biomaterials, and targeted drug delivery.

Bio

Ashutosh Chilkoti is the Alan L. Kaganov Professor of Biomedical Engineering and Chair of the Department of Biomedical Engineering at Duke University.

My research in biomolecular engineering and biointerface science focuses on the development of new molecular tools and technologies that borrow from molecular biology, protein engineering, polymer chemistry and surface science that we then exploit for the development of applications that span the range from bioseparations, plasmonic biosensors, low-cost clinical diagnostics, and drug delivery.

Education

Ph.D. University of Washington, 1991

Positions

Alan L. Kaganov Distinguished Professor of Biomedical Engineering
Professor of Biomedical Engineering
Professor in the Thomas Lord Department of Mechanical Engineering and Materials Science
Research Professor of Global Health
Professor in the Department of Chemistry
Core Faculty in Innovation & Entrepreneurship
Member of the Duke Cancer Institute

Awards, Honors, and Distinctions

Distinguished Alumnus Award. Indian Institute of Technology Delhi, India. 2015
Fellow (NAI). National Academy of Inventors. 2014
Pritzker Distinguished Lecture Award. Biomedical Engineering Society. 2013
Clemson Award for Contributions to the Literature. Society for Biomaterials. 2011
Clemson Award for Contributions to the Literature. Society for Biomaterials. 2011
Humboldt Senior Researcher Award. Alexander Von Humboldt Foundation. 2010
Stansell Family Distinguished Research Award. Duke University, Pratt School of Engineering. 2008
Fellow. American Institute for Medical and Biological Engineering. 1996

Courses Taught

MSEG 591: Independent Study
EGR 393: Research Projects in Engineering
EGR 391: Projects in Engineering
BME 792: Continuation of Graduate Independent Study
BME 791: Graduate Independent Study
BME 789: Internship in Biomedical Engineering
BME 570L: Introduction to Biomolecular Engineering (GE, BB, MC)
BME 494: Projects in Biomedical Engineering (GE)
BME 493: Projects in Biomedical Engineering (GE)
BME 394: Projects in Biomedical Engineering (GE)

Publications

Dai Y, Zhou Z, Yu W, Ma Y, Kim K, Rivera N, et al. Biomolecular condensates regulate cellular electrochemical equilibria. Cell. 2024 Sep;S0092-8674(24)00909-7.
Stormo BM, McLaughlin GA, Jalihal AP, Frederick LK, Cole SJ, Seim I, et al. Intrinsically disordered sequences can tune fungal growth and the cell cycle for specific temperatures. Curr Biol. 2024 Aug 19;34(16):3722-3734.e7.
Tang NC, Su JC, Shmidov Y, Kelly G, Deshpande S, Sirohi P, et al. Synthetic intrinsically disordered protein fusion tags that enhance protein solubility. Nature communications. 2024 May;15(1):3727.
Li Z, Shen Q, Usher ET, Anderson AP, Iburg M, Lin R, et al. Phase transition of GvpU regulates gas vesicle clustering in bacteria. Nature microbiology. 2024 Apr;9(4):1021–35.
Walter V, Schmatko T, Muller P, Schroder AP, MacEwan SR, Chilkoti A, et al. Negative lipid membranes enhance the adsorption of TAT-decorated elastin-like polypeptide micelles. Biophysical journal. 2024 Apr;123(7):901–8.
Albarghouthi FM, Semeniak D, Khanani I, Doherty JL, Smith BN, Salfity M, et al. Addressing Signal Drift and Screening for Detection of Biomarkers with Carbon Nanotube Transistors. ACS nano. 2024 Feb;
Strader RL, Shmidov Y, Chilkoti A. Encoding Structure in Intrinsically Disordered Protein Biomaterials. Accounts of chemical research. 2024 Feb;57(3):302–11.
Subramaniam S, Akay M, Anastasio MA, Bailey V, Boas D, Bonato P, et al. Grand Challenges at the Interface of Engineering and Medicine. IEEE Open J Eng Med Biol. 2024;5:1–13.
Saha S, Banskota S, Yousefpour P, Schaal JL, Zakharov N, Liu J, et al. Preclinical Development of a Genetically Engineered Albumin-Binding Nanoparticle of Paclitaxel. Small Science. 2024 Jan 1;
Deshpande S, Yang Y, Zauscher S, Chilkoti A. Enzymatic Synthesis of Aptamer-Polynucleotide Nanoparticles with High Anticancer Drug Loading for Targeted Delivery. Biomacromolecules. 2024 Jan;25(1):155–64.
Sethi V, Cohen-Gerassi D, Meir S, Ney M, Shmidov Y, Koren G, et al. Modulating hierarchical self-assembly in thermoresponsive intrinsically disordered proteins through high-temperature incubation time. Scientific reports. 2023 Dec;13(1):21688.
Lazar KM, Shetty S, Chilkoti A, Collier JH. Immune-active polymeric materials for the treatment of inflammatory diseases. Current Opinion in Colloid and Interface Science. 2023 Oct 1;67.
Semeniak D, Cruz DF, Chilkoti A, Mikkelsen MH. Plasmonic Fluorescence Enhancement in Diagnostics for Clinical Tests at Point-of-Care: A Review of Recent Technologies. Advanced materials (Deerfield Beach, Fla). 2023 Aug;35(34):e2107986.
Vahabi H, Liu J, Dai Y, Joh DY, Britton R, Heggestad J, et al. A gravity-driven droplet fluidic point-of-care test. Device. 2023 Jul;1(1):100009.
Kinnamon DS, Heggestad JT, Liu J, Nguyen T, Ly V, Hucknall AM, et al. Environmentally Resilient Microfluidic Point-of-Care Immunoassay Enables Rapid Diagnosis of Talaromycosis. ACS Sens. 2023 Jun 23;8(6):2228–36.
Burrow DT, Heggestad JT, Kinnamon DS, Chilkoti A. Engineering Innovative Interfaces for Point-of-Care Diagnostics. Current opinion in colloid & interface science. 2023 Jun;101718.
Dai Y, Chamberlayne CF, Messina MS, Chang CJ, Zare RN, You L, et al. Interface of biomolecular condensates modulates redox reactions. Chem. 2023 Jun;9(6):1594–609.
Heggestad JT, Britton RJ, Kinnamon DS, Liu J, Anderson JG, Joh DY, et al. COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray. Anal Chem. 2023 Apr 4;95(13):5610–7.
Dai Y, Farag M, Lee D, Zeng X, Kim K, Son H-I, et al. Programmable synthetic biomolecular condensates for cellular control. Nature chemical biology. 2023 Apr;19(4):518–28.
Ozer I, Slezak A, Sirohi P, Li X, Zakharov N, Yao Y, et al. An injectable PEG-like conjugate forms a subcutaneous depot and enables sustained delivery of a peptide drug. Biomaterials. 2023 Mar;294:121985.
Olson LB, Hunter NI, Rempel RE, Yu H, Spencer DM, Sullenger CZ, et al. Mixed-surface polyamidoamine polymer variants retain nucleic acid-scavenger ability with reduced toxicity. iScience. 2022 Dec 22;25(12):105542.
Schaal JL, Bhattacharyya J, Brownstein J, Strickland KC, Kelly G, Saha S, et al. Brachytherapy via a depot of biopolymer-bound 131I synergizes with nanoparticle paclitaxel in therapy-resistant pancreatic tumours. Nat Biomed Eng. 2022 Oct;6(10):1148–66.
Roden CA, Dai Y, Giannetti CA, Seim I, Lee M, Sealfon R, et al. Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures. Nucleic Acids Res. 2022 Aug 12;50(14):8168–92.
Saha S, Banskota S, Liu J, Zakharov N, Dzuricky M, Li X, et al. Genetically Engineered Nanoparticles of Asymmetric Triblock Polypeptide with a Platinum(IV) Cargo Outperforms a Platinum(II) Analog and Free Drug in a Murine Cancer Model. Nano Lett. 2022 Jul 27;22(14):5898–908.
Lin KH, Rutter JC, Xie A, Killarney ST, Vaganay C, Benaksas C, et al. P2RY2-AKT activation is a therapeutically actionable consequence of XPO1 inhibition in acute myeloid leukemia. Nat Cancer. 2022 Jul;3(7):837–51.
Kinnamon DS, Heggestad JT, Liu J, Chilkoti A. Technologies for Frugal and Sensitive Point-of-Care Immunoassays. Annual review of analytical chemistry (Palo Alto, Calif). 2022 Jun;15(1):123–49.
Ozer I, Kelly G, Gu R, Li X, Zakharov N, Sirohi P, et al. Polyethylene Glycol-Like Brush Polymer Conjugate of a Protein Drug Does Not Induce an Antipolymer Immune Response and Has Enhanced Pharmacokinetics than Its Polyethylene Glycol Counterpart. Adv Sci (Weinh). 2022 Apr;9(11):e2103672.
Milligan JJ, Saha S, Jenkins IC, Chilkoti A. Genetically encoded elastin-like polypeptide nanoparticles for drug delivery. Current opinion in biotechnology. 2022 Apr;74:146–53.
Kelly G, Milligan JJ, Mastria EM, Kim S, Zelenetz SR, Dobbins J, et al. Intratumoral delivery of brachytherapy and immunotherapy by a thermally triggered polypeptide depot. J Control Release. 2022 Mar;343:267–76.
Ozer I, Pitoc GA, Layzer JM, Moreno A, Olson LB, Layzer KD, et al. PEG-Like Brush Polymer Conjugate of RNA Aptamer That Shows Reversible Anticoagulant Activity and Minimal Immune Response. Adv Mater. 2022 Mar;34(10):e2107852.
Heggestad JT, Kinnamon DS, Liu J, Joh DY, Fontes CM, Wei Q, et al. Smartphone Enabled Point-of-Care Detection of Serum Biomarkers. Methods in molecular biology (Clifton, NJ). 2022 Jan;2393:343–65.
Heggestad JT, Britton RJ, Kinnamon DS, Wall SA, Joh DY, Hucknall AM, et al. Rapid test to assess the escape of SARS-CoV-2 variants of concern. Sci Adv. 2021 Dec 3;7(49):eabl7682.
Weber P, Dzuricky M, Min J, Jenkins I, Chilkoti A. Concentration-Independent Multivalent Targeting of Cancer Cells by Genetically Encoded Core-Crosslinked Elastin/Resilin-like Polypeptide Micelles. Biomacromolecules. 2021 Oct;22(10):4347–56.
Joh DY, Heggestad JT, Zhang S, Anderson GR, Bhattacharyya J, Wardell SE, et al. Author Correction: Cellphone enabled point-of-care assessment of breast tumor cytology and molecular HER2 expression from fine-needle aspirates. NPJ Breast Cancer. 2021 Sep 17;7(1):126.
Navarro LA, Ryan JJ, Dzuricky M, Gradzielski M, Chilkoti A, Zauscher S. Microphase Separation of Resilin-like and Elastin-like Diblock Copolypeptides in Concentrated Solutions. Biomacromolecules. 2021 Sep;22(9):3827–38.
Joh DY, Heggestad JT, Zhang S, Anderson GR, Bhattacharyya J, Wardell SE, et al. Cellphone enabled point-of-care assessment of breast tumor cytology and molecular HER2 expression from fine-needle aspirates. NPJ Breast Cancer. 2021 Jul 2;7(1):85.
Jenkins IC, Milligan JJ, Chilkoti A. Genetically Encoded Elastin-Like Polypeptides for Drug Delivery. Advanced healthcare materials. 2021 Jul;10(13):e2100209.
Shapiro DM, Ney M, Eghtesadi SA, Chilkoti A. Protein Phase Separation Arising from Intrinsic Disorder: First-Principles to Bespoke Applications. The journal of physical chemistry B. 2021 Jul;125(25):6740–59.
Heggestad JT, Kinnamon DS, Olson LB, Liu J, Kelly G, Wall SA, et al. Multiplexed, quantitative serological profiling of COVID-19 from blood by a point-of-care test. Sci Adv. 2021 Jun;7(26).
Fontes CM, Lipes BD, Liu J, Agans KN, Yan A, Shi P, et al. Ultrasensitive point-of-care immunoassay for secreted glycoprotein detects Ebola infection earlier than PCR. Sci Transl Med. 2021 Apr 7;13(588).
Hainline KM, Shores LS, Votaw NL, Bernstein ZJ, Kelly SH, Fries CN, et al. Modular complement assemblies for mitigating inflammatory conditions. Proceedings of the National Academy of Sciences of the United States of America. 2021 Apr;118(15):e2018627118.
Zeng X, Liu C, Fossat MJ, Ren P, Chilkoti A, Pappu RV. Design of intrinsically disordered proteins that undergo phase transitions with lower critical solution temperatures. APL materials. 2021 Feb;9(2):021119.
Laaß K, Quiroz FG, Hunold J, Roberts S, Chilkoti A, Hinderberger D. Nanoscopic Dynamics Dictate the Phase Separation Behavior of Intrinsically Disordered Proteins. Biomacromolecules. 2021 Feb;22(2):1015–25.
Li L, Shields CW, Huang J, Zhang Y, Ohiri KA, Yellen BB, et al. Rapid capture of biomolecules from blood via stimuli-responsive elastomeric particles for acoustofluidic separation. The Analyst. 2021 Jan;145(24):8087–96.
Yousefpour P, Varanko A, Subrahmanyan R, Chilkoti A. Recombinant Fusion of Glucagon-Like Peptide-1 and an Albumin Binding Domain Provides Glycemic Control for a Week in Diabetic Mice. Advanced Therapeutics. 2020 Oct 1;3(10).
Dodd RD, Scherer A, Huang W, McGivney GR, Gutierrez WR, Laverty EA, et al. Tumor Subtype Determines Therapeutic Response to Chimeric Polypeptide Nanoparticle-based Chemotherapy in Pten-deleted Mouse Models of Sarcoma. Clin Cancer Res. 2020 Sep 15;26(18):5036–47.
Dzuricky M, Rogers BA, Shahid A, Cremer PS, Chilkoti A. De novo engineering of intracellular condensates using artificial disordered proteins. Nature chemistry. 2020 Sep;12(9):814–25.
Gilroy CA, Capozzi ME, Varanko AK, Tong J, D’Alessio DA, Campbell JE, et al. Sustained release of a GLP-1 and FGF21 dual agonist from an injectable depot protects mice from obesity and hyperglycemia. Sci Adv. 2020 Aug;6(35):eaaz9890.
Zeng X, Holehouse AS, Chilkoti A, Mittag T, Pappu RV. Connecting Coil-to-Globule Transitions to Full Phase Diagrams for Intrinsically Disordered Proteins. Biophysical journal. 2020 Jul;119(2):402–18.
Cruz DF, Fontes CM, Semeniak D, Huang J, Hucknall A, Chilkoti A, et al. Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities. Nano letters. 2020 Jun;20(6):4330–6.
Varanko AK, Su JC, Chilkoti A. Elastin-Like Polypeptides for Biomedical Applications. Annual review of biomedical engineering. 2020 Jun;22:343–69.
Banskota S, Saha S, Bhattacharya J, Kirmani N, Yousefpour P, Dzuricky M, et al. Genetically Encoded Stealth Nanoparticles of a Zwitterionic Polypeptide-Paclitaxel Conjugate Have a Wider Therapeutic Window than Abraxane in Multiple Tumor Models. Nano Lett. 2020 Apr 8;20(4):2396–409.
Roberts S, Miao V, Costa S, Simon J, Kelly G, Shah T, et al. Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins. Nature communications. 2020 Mar;11(1):1342.
Williams NX, Watson N, Joh DY, Chilkoti A, Franklin AD. Aerosol jet printing of biological inks by ultrasonic delivery. Biofabrication. 2020 Feb;12(2):025004.
Varanko A, Saha S, Chilkoti A. Recent trends in protein and peptide-based biomaterials for advanced drug delivery. Advanced drug delivery reviews. 2020 Jan;156:133–87.
Lee JS, Hill RT, Chilkoti A, Murphy WL. Surface Patterning. In: Biomaterials Science: An Introduction to Materials in Medicine. 2020. p. 553–73.
Heggestad JT, Fontes CM, Joh DY, Hucknall AM, Chilkoti A. In Pursuit of Zero 2.0: Recent Developments in Nonfouling Polymer Brushes for Immunoassays. Advanced materials (Deerfield Beach, Fla). 2020 Jan;32(2):e1903285.
Wang J, Min J, Eghtesadi SA, Kane RS, Chilkoti A. Quantitative Study of the Interaction of Multivalent Ligand-Modified Nanoparticles with Breast Cancer Cells with Tunable Receptor Density. ACS nano. 2020 Jan;14(1):372–83.
Wang J, Saha S, Schaal JL, Yousefpour P, Li X, Chilkoti A. Heuristics for the Optimal Presentation of Bioactive Peptides on Polypeptide Micelles. Nano letters. 2019 Nov;19(11):7977–87.
Garcia Quiroz F, Li NK, Roberts S, Weber P, Dzuricky M, Weitzhandler I, et al. Intrinsically disordered proteins access a range of hysteretic phase separation behaviors. Science advances. 2019 Oct;5(10):eaax5177.
Willems L, van Westerveld L, Roberts S, Weitzhandler I, Calcines Cruz C, Hernandez-Garcia A, et al. Nature of Amorphous Hydrophilic Block Affects Self-Assembly of an Artificial Viral Coat Polypeptide. Biomacromolecules. 2019 Oct;20(10):3641–7.
Dai Z, Lee AJ, Roberts S, Sysoeva TA, Huang S, Dzuricky M, et al. Versatile biomanufacturing through stimulus-responsive cell-material feedback. Nature chemical biology. 2019 Oct;15(10):1017–24.
Manzari MT, Anderson GR, Lin KH, Soderquist RS, Çakir M, Zhang M, et al. Genomically informed small-molecule drugs overcome resistance to a sustained-release formulation of an engineered death receptor agonist in patient-derived tumor models. Sci Adv. 2019 Sep;5(9):eaaw9162.
Dzuricky M, Xiong S, Weber P, Chilkoti A. Avidity and Cell Uptake of Integrin-Targeting Polypeptide Micelles is Strongly Shape-Dependent. Nano letters. 2019 Sep;19(9):6124–32.
Simon JR, Eghtesadi SA, Dzuricky M, You L, Chilkoti A. Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells. Molecular cell. 2019 Jul;75(1):66-75.e5.
Varanko AK, Chilkoti A. Molecular and Materials Engineering for Delivery of Peptide Drugs to Treat Type 2 Diabetes. Advanced healthcare materials. 2019 Jun;8(12):e1801509.
Willems L, Roberts S, Weitzhandler I, Chilkoti A, Mastrobattista E, van der Oost J, et al. Inducible Fibril Formation of Silk-Elastin Diblocks. ACS omega. 2019 May;4(5):9135–43.
Koc J, Simovich T, Schönemann E, Chilkoti A, Gardner H, Swain GW, et al. Sediment challenge to promising ultra-low fouling hydrophilic surfaces in the marine environment. Biofouling. 2019 Apr;35(4):454–62.
Joh DY, Zimmers Z, Avlani M, Heggestad JT, Aydin HB, Ganson N, et al. Architectural Modification of Conformal PEG-Bottlebrush Coatings Minimizes Anti-PEG Antigenicity While Preserving Stealth Properties. Adv Healthc Mater. 2019 Apr;8(8):e1801177.
Yousefpour P, Ahn L, Tewksbury J, Saha S, Costa SA, Bellucci JJ, et al. Conjugate of Doxorubicin to Albumin-Binding Peptide Outperforms Aldoxorubicin. Small (Weinheim an der Bergstrasse, Germany). 2019 Mar;15(12):e1804452.
Fontes CM, Achar RK, Joh DY, Ozer I, Bhattacharjee S, Hucknall A, et al. Engineering the Surface Properties of a Zwitterionic Polymer Brush to Enable the Simple Fabrication of Inkjet-Printed Point-of-Care Immunoassays. Langmuir : the ACS journal of surfaces and colloids. 2019 Feb;35(5):1379–90.
Banskota S, Yousefpour P, Kirmani N, Li X, Chilkoti A. Long circulating genetically encoded intrinsically disordered zwitterionic polypeptides for drug delivery. Biomaterials. 2019 Feb;192:475–85.
Deshpande S, Yang Y, Chilkoti A, Zauscher S. Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase. In 2019. p. 163–88.
Mozhdehi D, Luginbuhl KM, Dzuricky M, Costa SA, Xiong S, Huang FC, et al. Genetically Encoded Cholesterol-Modified Polypeptides. Journal of the American Chemical Society. 2019 Jan;141(2):945–51.
Costa SA, Mozhdehi D, Dzuricky MJ, Isaacs FJ, Brustad EM, Chilkoti A. Active Targeting of Cancer Cells by Nanobody Decorated Polypeptide Micelle with Bio-orthogonally Conjugated Drug. Nano letters. 2019 Jan;19(1):247–54.
Roberts S, Harmon TS, Schaal JL, Miao V, Li KJ, Hunt A, et al. Author Correction: Injectable tissue integrating networks from recombinant polypeptides with tunable order. Nat Mater. 2018 Dec;17(12):1164.
Yousefpour P, McDaniel JR, Prasad V, Ahn L, Li X, Subrahmanyan R, et al. Genetically Encoding Albumin Binding into Chemotherapeutic-loaded Polypeptide Nanoparticles Enhances Their Antitumor Efficacy. Nano letters. 2018 Dec;18(12):7784–93.
Roberts S, Harmon TS, Schaal JL, Miao V, Li KJ, Hunt A, et al. Injectable tissue integrating networks from recombinant polypeptides with tunable order. Nat Mater. 2018 Dec;17(12):1154–63.
Ruff KM, Roberts S, Chilkoti A, Pappu RV. Advances in Understanding Stimulus-Responsive Phase Behavior of Intrinsically Disordered Protein Polymers. Journal of molecular biology. 2018 Nov;430(23):4619–35.
Gu R, Oweida T, Yingling YG, Chilkoti A, Zauscher S. Enzymatic Synthesis of Nucleobase-Modified Single-Stranded DNA Offers Tunable Resistance to Nuclease Degradation. Biomacromolecules. 2018 Aug;19(8):3525–35.
Li NK, Roberts S, Quiroz FG, Chilkoti A, Yingling YG. Sequence Directionality Dramatically Affects LCST Behavior of Elastin-Like Polypeptides. Biomacromolecules. 2018 Jul;19(7):2496–505.
Dzuricky M, Roberts S, Chilkoti A. Convergence of Artificial Protein Polymers and Intrinsically Disordered Proteins. Biochemistry. 2018 May;57(17):2405–14.
Gilroy CA, Roberts S, Chilkoti A. Fusion of fibroblast growth factor 21 to a thermally responsive biopolymer forms an injectable depot with sustained anti-diabetic action. Journal of controlled release : official journal of the Controlled Release Society. 2018 May;277:154–64.
Mozhdehi D, Luginbuhl KM, Simon JR, Dzuricky M, Berger R, Varol HS, et al. Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly. Nature chemistry. 2018 May;10(5):496–505.
Costa SA, Simon JR, Amiram M, Tang L, Zauscher S, Brustad EM, et al. Photo-Crosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Microgels of Intrinsically Disordered Polypeptides. Advanced materials (Deerfield Beach, Fla). 2018 Feb;30(5).
Li L, Li NK, Tu Q, Im O, Mo C-K, Han W, et al. Functional Modification of Silica through Enhanced Adsorption of Elastin-Like Polypeptide Block Copolymers. Biomacromolecules. 2018 Feb;19(2):298–306.
Mastria EM, Cai LY, Kan MJ, Li X, Schaal JL, Fiering S, et al. Nanoparticle formulation improves doxorubicin efficacy by enhancing host antitumor immunity. J Control Release. 2018 Jan 10;269:364–73.
Luginbuhl KM, Mozhdehi D, Dzuricky M, Yousefpour P, Huang FC, Mayne NR, et al. Recombinant Synthesis of Hybrid Lipid-Peptide Polymer Fusions that Self-Assemble and Encapsulate Hydrophobic Drugs. Angewandte Chemie (International ed in English). 2017 Nov;56(45):13979–84.
Wang J, Dzuricky M, Chilkoti A. The Weak Link: Optimization of the Ligand-Nanoparticle Interface To Enhance Cancer Cell Targeting by Polymer Micelles. Nano letters. 2017 Oct;17(10):5995–6005.
Correction for Joh et al., Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood. Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):E8315.
Bhattacharyya J, Ren X-R, Mook RA, Wang J, Spasojevic I, Premont RT, et al. Niclosamide-conjugated polypeptide nanoparticles inhibit Wnt signaling and colon cancer growth. Nanoscale. 2017 Aug 31;9(34):12709–17.
Wang J, Bhattacharyya J, Mastria E, Chilkoti A. A quantitative study of the intracellular fate of pH-responsive doxorubicin-polypeptide nanoparticles. J Control Release. 2017 Aug 28;260:100–10.
Joh DY, Hucknall AM, Wei Q, Mason KA, Lund ML, Fontes CM, et al. Inkjet-printed point-of-care immunoassay on a nanoscale polymer brush enables subpicomolar detection of analytes in blood. Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):E7054–62.
Weitzhandler I, Dzuricky M, Hoffmann I, Garcia Quiroz F, Gradzielski M, Chilkoti A. Micellar Self-Assembly of Recombinant Resilin-/Elastin-Like Block Copolypeptides. Biomacromolecules. 2017 Aug;18(8):2419–26.
MacEwan SR, Chilkoti A. From Composition to Cure: A Systems Engineering Approach to Anticancer Drug Carriers. Angewandte Chemie (International ed in English). 2017 Jun;56(24):6712–33.
Tang L, Navarro LA, Chilkoti A, Zauscher S. High-Molecular-Weight Polynucleotides by Transferase-Catalyzed Living Chain-Growth Polycondensation. Angewandte Chemie (International ed in English). 2017 Jun;56(24):6778–82.
Han W, Chilkoti A, López GP. Self-assembled hybrid elastin-like polypeptide/silica nanoparticles enable triggered drug release. Nanoscale. 2017 May;9(18):6178–86.
Bhattacharyya J, Weitzhandler I, Ho SB, McDaniel JR, Li X, Tang L, et al. Encapsulating a Hydrophilic Chemotherapeutic into Rod-like Nanoparticles of a Genetically Encoded Asymmetric Triblock Polypeptide Improves its Efficacy. Adv Funct Mater. 2017 Mar 24;27(12).
Weinberger A, Walter V, MacEwan SR, Schmatko T, Muller P, Schroder AP, et al. Cargo self-assembly rescues affinity of cell-penetrating peptides to lipid membranes. Scientific Reports. 2017 Mar 6;7.
Ozer I, Chilkoti A. Site-Specific and Stoichiometric Stealth Polymer Conjugates of Therapeutic Peptides and Proteins. Bioconjugate chemistry. 2017 Mar;28(3):713–23.
Widder K, MacEwan SR, Garanger E, Núñez V, Lecommandoux S, Chilkoti A, et al. Characterisation of hydration and nanophase separation during the temperature response in hydrophobic/hydrophilic elastin-like polypeptide (ELP) diblock copolymers. Soft matter. 2017 Mar;13(9):1816–22.
MacEwan SR, Weitzhandler I, Hoffmann I, Genzer J, Gradzielski M, Chilkoti A. Phase Behavior and Self-Assembly of Perfectly Sequence-Defined and Monodisperse Multiblock Copolypeptides. Biomacromolecules. 2017 Feb;18(2):599–609.
Joh DY, McGuire F, Abedini-Nassab R, Andrews JB, Achar RK, Zimmers Z, et al. Poly(oligo(ethylene glycol) methyl ether methacrylate) Brushes on High-κ Metal Oxide Dielectric Surfaces for Bioelectrical Environments. ACS applied materials & interfaces. 2017 Feb;9(6):5522–9.
Wang J, MacEwan SR, Chilkoti A. Quantitative Mapping of the Spatial Distribution of Nanoparticles in Endo-Lysosomes by Local pH. Nano letters. 2017 Feb;17(2):1226–32.
Luginbuhl KM, Schaal JL, Umstead B, Mastria EM, Li X, Banskota S, et al. One-week glucose control via zero-order release kinetics from an injectable depot of glucagon-like peptide-1 fused to a thermosensitive biopolymer. Nat Biomed Eng. 2017;1.
Banskota S, Yousefpour P, Chilkoti A. Cell-Based Biohybrid Drug Delivery Systems: The Best of the Synthetic and Natural Worlds. Macromolecular bioscience. 2017 Jan;17(1).
Roberts S, Costa S, Schaal J, Simon JR, Dzuricky M, Quiroz FG, et al. 2.5 Elastin-like polypeptides. In: Comprehensive Biomaterials II. 2017. p. 90–108.
Simon JR, Carroll NJ, Rubinstein M, Chilkoti A, López GP. Programming molecular self-assembly of intrinsically disordered proteins containing sequences of low complexity. Nature Chemistry. 2017;
Gonzalez MA, Simon JR, Ghoorchian A, Scholl Z, Lin S, Rubinstein M, et al. Strong, Tough, Stretchable, and Self-Adhesive Hydrogels from Intrinsically Unstructured Proteins. Advanced Materials. 2017;
Kwok KWH, Dong W, Marinakos SM, Liu J, Chilkoti A, Wiesner MR, et al. Silver nanoparticle toxicity is related to coating materials and disruption of sodium concentration regulation. Nanotoxicology. 2016 Nov;10(9):1306–17.
Abedini-Nassab R, Joh DY, Albarghouthi F, Chilkoti A, Murdoch DM, Yellen BB. Magnetophoretic transistors in a tri-axial magnetic field. Lab Chip. 2016 Oct 18;16(21):4181–8.
Gilroy CA, Luginbuhl KM, Chilkoti A. Controlled release of biologics for the treatment of type 2 diabetes. Journal of controlled release : official journal of the Controlled Release Society. 2016 Oct;240:151–64.
Pham AT, Seto R, Schönke J, Joh DY, Chilkoti A, Fried E, et al. Crystallization kinetics of binary colloidal monolayers. Soft matter. 2016 Oct;12(37):7735–46.
Pang Y, Liu J, Qi Y, Li X, Chilkoti A. A Modular Method for the High-Yield Synthesis of Site-Specific Protein-Polymer Therapeutics. Angewandte Chemie (International ed in English). 2016 Aug;55(35):10296–300.
Liu J, Pang Y, Bhattacharyya J, Liu W, Weitzhandler I, Li X, et al. Developing Precisely Defined Drug-Loaded Nanoparticles by Ring-Opening Polymerization of a Paclitaxel Prodrug. Advanced healthcare materials. 2016 Aug;5(15):1868–73.
Abedini-Nassab R, Joh DY, Van Heest M, Baker C, Chilkoti A, Murdoch DM, et al. Magnetophoretic Conductors and Diodes in a 3D Magnetic Field. Adv Funct Mater. 2016 Jun 14;26(22):4026–34.
Li L, Mo C-K, Chilkoti A, Lopez GP, Carroll NJ. Creating cellular patterns using genetically engineered, gold- and cell-binding polypeptides. Biointerphases. 2016 Jun;11(2):021009.
Schaal JL, Li X, Mastria E, Bhattacharyya J, Zalutsky MR, Chilkoti A, et al. Injectable polypeptide micelles that form radiation crosslinked hydrogels in situ for intratumoral radiotherapy. J Control Release. 2016 Apr 28;228:58–66.
Tang NC, Chilkoti A. Combinatorial codon scrambling enables scalable gene synthesis and amplification of repetitive proteins. Nature materials. 2016 Apr;15(4):419–24.
Nawroth JF, McDaniel JR, Chilkoti A, Jordan R, Luxenhofer R. Maleimide-Functionalized Poly(2-Oxazoline)s and Their Conjugation to Elastin-Like Polypeptides. Macromolecular bioscience. 2016 Mar;16(3):322–33.
Mukerji R, Schaal J, Li X, Bhattacharyya J, Asai D, Zalutsky MR, et al. Spatiotemporally photoradiation-controlled intratumoral depot for combination of brachytherapy and photodynamic therapy for solid tumor. Biomaterials. 2016 Feb;79:79–87.
Qi Y, Simakova A, Ganson NJ, Li X, Luginbuhl KM, Özer I, et al. A brush-polymer conjugate of exendin-4 reduces blood glucose for up to five days and eliminates poly(ethylene glycol) antigenicity. Nat Biomed Eng. 2016;1.
Bhattacharyya J, Bellucci JJ, Chilkoti A. Elastin-Like Polypeptides: Bio-Inspired Smart Polymers for Protein Purification, Drug Delivery and Tissue Engineering. In: Biomaterials from Nature for Advanced Devices and Therapies. 2016. p. 106–26.
Bhattacharjee S, Liu W, Wang W-H, Weitzhandler I, Li X, Qi Y, et al. Site-Specific Zwitterionic Polymer Conjugates of a Protein Have Long Plasma Circulation. Chembiochem : a European journal of chemical biology. 2015 Nov;16(17):2451–5.
Li NK, Fuss WH, Tang L, Gu R, Chilkoti A, Zauscher S, et al. Prediction of solvent-induced morphological changes of polyelectrolyte diblock copolymer micelles. Soft matter. 2015 Nov;11(42):8236–45.
Quiroz FG, Chilkoti A. Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers. Nature materials. 2015 Nov;14(11):1164–71.
Abedini-Nassab R, Joh DY, Van Heest MA, Yi JS, Baker C, Taherifard Z, et al. Characterizing the Switching Thresholds of Magnetophoretic Transistors. Adv Mater. 2015 Oct 28;27(40):6176–80.
Qi Y, Chilkoti A. Protein-polymer conjugation-moving beyond PEGylation. Current opinion in chemical biology. 2015 Oct;28:181–93.
Garanger E, MacEwan SR, Sandre O, Brûlet A, Bataille L, Chilkoti A, et al. Structural Evolution of a Stimulus-Responsive Diblock Polypeptide Micelle by Temperature Tunable Compaction of its Core. Macromolecules. 2015 Sep 22;48(18):6617–27.
Roberts S, Dzuricky M, Chilkoti A. Elastin-like polypeptides as models of intrinsically disordered proteins. FEBS letters. 2015 Sep;589(19 Pt A):2477–86.
Bhattacharyya J, Bellucci JJ, Weitzhandler I, McDaniel JR, Spasojevic I, Li X, et al. A paclitaxel-loaded recombinant polypeptide nanoparticle outperforms Abraxane in multiple murine cancer models. Nat Commun. 2015 Aug 4;6:7939.
Han W, MacEwan SR, Chilkoti A, López GP. Bio-inspired synthesis of hybrid silica nanoparticles templated from elastin-like polypeptide micelles. Nanoscale. 2015 Jul;7(28):12038–44.
Mastria EM, Chen M, McDaniel JR, Li X, Hyun J, Dewhirst MW, et al. Doxorubicin-conjugated polypeptide nanoparticles inhibit metastasis in two murine models of carcinoma. J Control Release. 2015 Jun 28;208:52–8.
Ghoorchian A, Simon JR, Bharti B, Han W, Zhao X, Chilkoti A, et al. Bioinspired reversibly cross-linked hydrogels comprising polypeptide micelles exhibit enhanced mechanical properties. Advanced Functional Materials. 2015 Jun 1;25(21):3122–30.
Kimmerling KA, Furman BD, Mangiapani DS, Moverman MA, Sinclair SM, Huebner JL, et al. Sustained intra-articular delivery of IL-1RA from a thermally-responsive elastin-like polypeptide as a therapy for post-traumatic arthritis. Eur Cell Mater. 2015 Jan 31;29:124–39.
Bellucci JJ, Bhattacharyya J, Chilkoti A. A noncanonical function of sortase enables site-specific conjugation of small molecules to lysine residues in proteins. Angewandte Chemie (International ed in English). 2015 Jan;54(2):441–5.
Liu J, Liu W, Weitzhandler I, Bhattacharyya J, Li X, Wang J, et al. Ring-opening polymerization of prodrugs: a versatile approach to prepare well-defined drug-loaded nanoparticles. Angewandte Chemie (International ed in English). 2015 Jan;54(3):1002–6.
Hassouneh W, Zhulina EB, Chilkoti A, Rubinstein M. Elastin-like Polypeptide Diblock Copolymers Self-Assemble into Weak Micelles. Macromolecules. 2015;
McDaniel JR, Weitzhandler I, Prevost S, Vargo KB, Appavou M-S, Hammer DA, et al. Noncanonical self-assembly of highly asymmetric genetically encoded polypeptide amphiphiles into cylindrical micelles. Nano letters. 2014 Nov;14(11):6590–8.
Liu W, Mukerji R, Li X, Schaal J, Bhattacharyya J, Zalutsky M, et al. Abstract 4583: In situ photocontrolled intratumoral depot for combined photodynamic therapy and brachytherapy for solid tumor. In: Cancer Research. American Association for Cancer Research (AACR); 2014. p. 4583–4583.
Li NK, García Quiroz F, Hall CK, Chilkoti A, Yingling YG. Molecular description of the LCST behavior of an elastin-like polypeptide. Biomacromolecules. 2014 Oct;15(10):3522–30.
Bhattacharyya J, Chilkoti A. Abstract 4481: Genetically encoded chimeric polypeptide nanoparticles for gemcitabine delivery to solid tumors. In: Cancer Research. American Association for Cancer Research (AACR); 2014. p. 4481–4481.
Hill RT, Kozek KM, Hucknall A, Smith DR, Chilkoti A. Nanoparticle-Film Plasmon Ruler Interrogated with Transmission Visible Spectroscopy. ACS photonics. 2014 Oct;1(10):974–84.
MacEwan SR, Chilkoti A. Applications of elastin-like polypeptides in drug delivery. Journal of controlled release : official journal of the Controlled Release Society. 2014 Sep;190:314–30.
Yousefpour P, Chilkoti A. Co-opting biology to deliver drugs. Biotechnology and bioengineering. 2014 Sep;111(9):1699–716.
Chilkoti A. Surface initiated polymerization and its applications in medicine. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2014.
Ghoorchian A, Chilkoti A, López GP. Simple assay for proteases based on aggregation of stimulus-responsive polypeptides. Analytical chemistry. 2014 Jun;86(12):6103–10.
MacEwan SR, Hassouneh W, Chilkoti A. Non-chromatographic purification of recombinant elastin-like polypeptides and their fusions with peptides and proteins from Escherichia coli. Journal of visualized experiments : JoVE. 2014 Jun;(88).
McDaniel JR, MacEwan SR, Li X, Radford DC, Landon CD, Dewhirst M, et al. Rational design of "heat seeking" drug loaded polypeptide nanoparticles that thermally target solid tumors. Nano Lett. 2014 May 14;14(5):2890–5.
Tang L, Tjong V, Li N, Yingling YG, Chilkoti A, Zauscher S. Enzymatic polymerization of high molecular weight DNA amphiphiles that self-assemble into star-like micelles. Advanced materials (Deerfield Beach, Fla). 2014 May;26(19):3050–4.
Chilkoti A. Genetically encoded polypeptide nanoparticles for delivery of drugs. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2014.
Tjong V, Tang L, Zauscher S, Chilkoti A. "Smart" DNA interfaces. Chemical Society reviews. 2014 Mar;43(5):1612–26.
Mastria E, Chilkoti A. Genetically encoded smart peptide polymers for biomedicine. MRS Bulletin. 2014 Jan 27;39(1):35–43.
Qi Y, Chilkoti A. Growing polymers from peptides and proteins: A biomedical perspective. Polymer Chemistry. 2014 Jan 21;5(2):266–76.
MacEwan SR, Hassouneh W, Chilkoti A. Non-chromatographic purification of recombinant elastin-like polypeptides and their fusions with peptides and proteins from Escherichia coli. Journal of visualized experiments : JoVE. 2014 Jan 1;(88).
Quiroz FG, Chilkoti A. The language of protein polymers. In: ACS Symposium Series. 2014. p. 15–33.
MacEwan SR, Chilkoti A. Controlled apoptosis by a thermally toggled nanoscale amplifier of cellular uptake. Nano letters. 2014 Jan;14(4):2058–64.
Li L, Im O, Ashutosh C, López GP. Encapsulation of stimuli-responsive fusion proteins in silica: Thermally responsive metal ion-sensitive hybrid membranes. Materials Research Society Symposium Proceedings. 2013 Dec 1;1498:169–75.
Qi Y, Amiram M, Gao W, Chilkoti A. Sortase-mediated initiator attachment for in situ grafting of a PEG-based polymer brush from the C terminus of green fluorescent protein. J Control Release. 2013 Nov 28;172(1):e121.
Amiram M, Luginbuhl KM, Li X, Feinglos MN, Chilkoti A. A depot-forming glucagon-like peptide-1 fusion protein reduces blood glucose for five days with a single injection. Journal of controlled release : official journal of the Controlled Release Society. 2013 Nov;172(1):144–51.
Sinclair SM, Bhattacharyya J, McDaniel JR, Gooden DM, Gopalaswamy R, Chilkoti A, et al. A genetically engineered thermally responsive sustained release curcumin depot to treat neuroinflammation. Journal of controlled release : official journal of the Controlled Release Society. 2013 Oct;171(1):38–47.
Tang L, Tjong V, Gu R, Chilkoti A, Zauscher S. Enzyme catalyzed polymerization of DNA amphiphiles that self-assemble into star-like micelles. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2013.
Chilkoti A. Genetically engineered polypeptide conjugates and fusions for drug delivery. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2013.
Garanger E, Mac Ewan S, Brulet A, Bataille L, Sandre O, Chilkoti A, et al. Elastin-based amphiphilic copolymers as precision building blocks for controlled self-assembly. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2013.
Chilkoti A. Syntax of "smart" peptide polymers governs their function. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2013.
McDaniel JR, Dewhirst MW, Chilkoti A. Actively targeting solid tumours with thermoresponsive drug delivery systems that respond to mild hyperthermia. Int J Hyperthermia. 2013 Sep;29(6):501–10.
Tjong V, Yu H, Hucknall A, Chilkoti A. Amplified fluorescence detection of DNA and RNA hybridization by surface initiated enzymatic polymerization (SIEP). Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. 2013 Aug 9;3:32–6.
McDaniel JR, Radford DC, Chilkoti A. A unified model for de novo design of elastin-like polypeptides with tunable inverse transition temperatures. Biomacromolecules. 2013 Aug;14(8):2866–72.
Qi Y, Amiram M, Gao W, McCafferty DG, Chilkoti A. Sortase-catalyzed initiator attachment enables high yield growth of a stealth polymer from the C terminus of a protein. Macromolecular rapid communications. 2013 Aug;34(15):1256–60.
Khan MN, Tjong V, Chilkoti A, Zharnikov M. Spectroscopic study of a DNA brush synthesized in situ by surface initiated enzymatic polymerization. The journal of physical chemistry B. 2013 Aug;117(34):9929–38.
Weinberger A, Macewan S, Schmatko T, Schroder A, Chilkoti A, Marques CM. Interactions of elastin-like polypeptides with model membranes on GUVs. In: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. SPRINGER; 2013. p. S158–S158.
Hassouneh W, Nunalee ML, Shelton MC, Chilkoti A. Calcium binding peptide motifs from calmodulin confer divalent ion selectivity to elastin-like polypeptides. Biomacromolecules. 2013 Jul;14(7):2347–53.
Kurzbach D, Hassouneh W, McDaniel JR, Jaumann EA, Chilkoti A, Hinderberger D. Hydration layer coupling and cooperativity in phase behavior of stimulus responsive peptide polymers. Journal of the American Chemical Society. 2013 Jul;135(30):11299–308.
Weller D, McDaniel JR, Fischer K, Chilkoti A, Schmidt M. Cylindrical polymer brushes with elastin-like polypeptide side chains. Macromolecules. 2013 Jun 25;46(12):4966–71.
Christensen T, Hassouneh W, Trabbic-Carlson K, Chilkoti A. Predicting transition temperatures of elastin-like polypeptide fusion proteins. Biomacromolecules. 2013 May;14(5):1514–9.
Bhattacharyya J, McDaniel J, Chilkoti A. Abstract 4509: Genetically encoded chimeric polypeptide nanoparticles for paclitaxel delivery to triple negative breast cancer. In: Cancer Research. American Association for Cancer Research (AACR); 2013. p. 4509–4509.
Liu W, McDaniel JR, Li X, Schaal J, Bhattacharyya J, Zalutsky MR, et al. Abstract 4341: A novel injectable polymer liquid that self-assembles into radioactive “seeds” for brachytherapy. In: Cancer Research. American Association for Cancer Research (AACR); 2013. p. 4341–4341.
Schaal JL, Liu W, Li X, Zalutsky M, Chilkoti A. Abstract 5615: Evaluation of the antitumoral efficacy and toxicity of injectable, polymeric radionuclide seeds in a noninvasive preclinical prostate tumor model. In: Cancer Research. American Association for Cancer Research (AACR); 2013. p. 5615–5615.
Caudill C, Bhattacharyya J, Chilkoti A. Delivery of paclitaxel with elastin-like polypeptides. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2013.
Sinclair SM, Bhattacharyya J, Little D, Bowles RD, Gooden DM, Toone EJ, et al. Pharmacokinetics of a thermally responsive curcumin conjugate for local antagonism of neuroinflammation in disc herniation. In: Osteoarthritis and Cartilage. Elsevier BV; 2013. p. S292–3.
Bellucci JJ, Amiram M, Bhattacharyya J, McCafferty D, Chilkoti A. Three-in-one chromatography-free purification, tag removal, and site-specific modification of recombinant fusion proteins using sortase A and elastin-like polypeptides. Angewandte Chemie (International ed in English). 2013 Mar;52(13):3703–8.
McDaniel JR, Bhattacharyya J, Vargo KB, Hassouneh W, Hammer DA, Chilkoti A. Self-assembly of thermally responsive nanoparticles of a genetically encoded peptide polymer by drug conjugation. Angewandte Chemie (International ed in English). 2013 Feb;52(6):1683–7.
Amiram M, Luginbuhl KM, Li X, Feinglos MN, Chilkoti A. Injectable protease-operated depots of glucagon-like peptide-1 provide extended and tunable glucose control. Proceedings of the National Academy of Sciences of the United States of America. 2013 Feb;110(8):2792–7.
Tjong V, Yu H, Hucknall A, Chilkoti A. Direct fluorescence detection of RNA on microarrays by surface-initiated enzymatic polymerization. Analytical chemistry. 2013 Jan;85(1):426–33.
Hill RT, Chilkoti A. Surface Patterning. In: Biomaterials Science: An Introduction to Materials: Third Edition. 2013. p. 276–301.
Lassiter JB, McGuire F, Mock JJ, Ciracì C, Hill RT, Wiley BJ, et al. Plasmonic waveguide modes of film-coupled metallic nanocubes. Nano letters. 2013 Jan;13(12):5866–72.
Thapa A, Han W, Simons RH, Chilkoti A, Chi EY, López GP. Effect of detergents on the thermal behavior of elastin-like polypeptides. Biopolymers. 2013 Jan;99(1):55–62.
MacEwan SR, Chilkoti A. Harnessing the power of cell-penetrating peptides: activatable carriers for targeting systemic delivery of cancer therapeutics and imaging agents. Wiley interdisciplinary reviews Nanomedicine and nanobiotechnology. 2013 Jan;5(1):31–48.
Moreau A, Ciracì C, Mock JJ, Hill RT, Wang Q, Wiley BJ, et al. Controlled-reflectance surfaces with film-coupled colloidal nanoantennas. Nature. 2012 Dec;492(7427):86–9.
Liu W, McDaniel J, Li X, Asai D, Quiroz FG, Schaal J, et al. Brachytherapy using injectable seeds that are self-assembled from genetically encoded polypeptides in situ. Cancer Res. 2012 Nov 15;72(22):5956–65.
Manzoor AA, Lindner LH, Landon CD, Park J-Y, Simnick AJ, Dreher MR, et al. Overcoming limitations in nanoparticle drug delivery: triggered, intravascular release to improve drug penetration into tumors. Cancer Res. 2012 Nov 1;72(21):5566–75.
Khan MN, Tjong V, Chilkoti A, Zharnikov M. Fabrication of ssDNA/oligo(ethylene glycol) monolayers and complex nanostructures by an irradiation-promoted exchange reaction. Angewandte Chemie (International ed in English). 2012 Oct;51(41):10303–6.
Hill RT, Mock JJ, Hucknall A, Wolter SD, Jokerst NM, Smith DR, et al. Plasmon ruler with angstrom length resolution. ACS nano. 2012 Oct;6(10):9237–46.
Kwok KWH, Auffan M, Badireddy AR, Nelson CM, Wiesner MR, Chilkoti A, et al. Uptake of silver nanoparticles and toxicity to early life stages of Japanese medaka (Oryzias latipes): effect of coating materials. Aquatic toxicology (Amsterdam, Netherlands). 2012 Sep;120–121:59–66.
Chilkoti A. Biomolecular toolbox for the in situ synthesis of protein-polymer conjugates. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2012.
Ciracì C, Hill RT, Mock JJ, Urzhumov Y, Fernández-Domínguez AI, Maier SA, et al. Probing the ultimate limits of plasmonic enhancement. Science (New York, NY). 2012 Aug;337(6098):1072–4.
Xu D, Asai D, Chilkoti A, Craig SL. Rheological properties of cysteine-containing elastin-like polypeptide solutions and hydrogels. Biomacromolecules. 2012 Aug;13(8):2315–21.
Asai D, Xu D, Liu W, Garcia Quiroz F, Callahan DJ, Zalutsky MR, et al. Protein polymer hydrogels by in situ, rapid and reversible self-gelation. Biomaterials. 2012 Jul;33(21):5451–8.
Hubbell JA, Chilkoti A. Chemistry. Nanomaterials for drug delivery. Science (New York, NY). 2012 Jul;337(6092):303–5.
Macewan SR, Chilkoti A. Digital switching of local arginine density in a genetically encoded self-assembled polypeptide nanoparticle controls cellular uptake. Nano letters. 2012 Jun;12(6):3322–8.
McDaniel JR, Macewan SR, Dewhirst M, Chilkoti A. Doxorubicin-conjugated chimeric polypeptide nanoparticles that respond to mild hyperthermia. J Control Release. 2012 May 10;159(3):362–7.
Hassouneh W, Fischer K, MacEwan SR, Branscheid R, Fu CL, Liu R, et al. Unexpected multivalent display of proteins by temperature triggered self-assembly of elastin-like polypeptide block copolymers. Biomacromolecules. 2012 May;13(5):1598–605.
Mock JJ, Hill RT, Tsai Y-J, Chilkoti A, Smith DR. Probing dynamically tunable localized surface plasmon resonances of film-coupled nanoparticles by evanescent wave excitation. Nano letters. 2012 Apr;12(4):1757–64.
Callahan DJ, Liu W, Li X, Dreher MR, Hassouneh W, Kim M, et al. Triple stimulus-responsive polypeptide nanoparticles that enhance intratumoral spatial distribution. Nano letters. 2012 Apr;12(4):2165–70.
Chilkoti A. Genetically encoded stimulus responsive elastin-like polypeptides: Applications in drug delivery. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2012.
Fluegel S, Kiefer T, Heimann N, Buehler J, Fischer K, McDaniel J, et al. Conjugates of polypeptides and synthetic polymers with oligonucleotides: Stars, micelles and multiblockcopolymers. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2012.
Tjong V, Zhang J, Chilkoti A, Zauscher S. Stimulus-Responsive Polymers as Intelligent Coatings for Biosensors: Architectures, Response Mechanisms, and Applications. 2012 Feb 3;1–30.
Ferris R, Hucknall A, Kwon BS, Chilkoti A, Zauscher S. Field-induced nanolithography for the patterning of non-fouling polymer brushes. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2012.
Hassouneh W, MacEwan SR, Chilkoti A. Fusions of elastin-like polypeptides to pharmaceutical proteins. Methods in enzymology. 2012 Jan;502:215–37.
Christensen T, Hassouneh W, Callahan DJ, Chilkoti A. Protein switches. 2012 Jan 1;3:238–66.
Nusz G, Chilkoti A. Individual plasmonic nanostructures as label free biosensors. In: Nanoplasmonic Sensors. 2012. p. 105–26.
Chen SY, Mock JJ, Hill RT, Chilkoti A, Smith DR, Lazarides AA. Raman antenna formed by molecule/plasmonic nanostructure hybrid system. Optics InfoBase Conference Papers. 2011 Dec 1;
Seekell K, Crow MJ, Marinakos S, Ostrander J, Chilkoti A, Wax A. Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles. Journal of biomedical optics. 2011 Nov;16(11):116003.
Ferris R, Hucknall A, Kwon BS, Chen T, Chilkoti A, Zauscher S. Field-induced nanolithography for patterning of non-fouling polymer brush surfaces. Small (Weinheim an der Bergstrasse, Germany). 2011 Nov;7(21):3032–7.
Simnick AJ, Amiram M, Liu W, Hanna G, Dewhirst MW, Kontos CD, et al. In vivo tumor targeting by a NGR-decorated micelle of a recombinant diblock copolypeptide. J Control Release. 2011 Oct 30;155(2):144–51.
Chen SY, Mock JJ, Hill RT, Chilkoti A, Smith DR, Lazarides AA. Raman antenna formed by molecule/plasmonic nanostructure hybrid system. 2011 Conference on Lasers and Electro-Optics: Laser Science to Photonic Applications, CLEO 2011. 2011 Sep 1;
Nusz GJ, Marinakos SM, Rangarajan S, Chilkoti A. Dual-order snapshot spectral imaging of plasmonic nanoparticles. Applied optics. 2011 Jul;50(21):4198–206.
Tjong V, Yu H, Hucknall A, Rangarajan S, Chilkoti A. Amplified on-chip fluorescence detection of DNA hybridization by surface-initiated enzymatic polymerization. Analytical chemistry. 2011 Jul;83(13):5153–9.
Gao W, Xu D, Lim DW, Craig SL, Chilkoti A. In situ growth of a thermoresponsive polymer from a genetically engineered elastin-like polypeptide. Polymer Chemistry. 2011 Jul 1;2(7):1561–6.
Fluegel S, Buehler J, Fischer K, McDaniel JR, Chilkoti A, Schmidt M. Self-assembly of monodisperse oligonucleotide-elastin block copolymers into stars and compound micelles. Chemistry (Weinheim an der Bergstrasse, Germany). 2011 May;17(20):5503–6.
Crow MJ, Seekell K, Marinakos S, Ostrander J, Chilkoti A, Wax AP. Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2011 Apr 29;7911.
Ahmad SA, Leggett GJ, Hucknall A, Chilkoti A. Micro- and nanostructured poly[oligo(ethylene glycol)methacrylate] brushes grown from photopatterned halogen initiators by atom transfer radical polymerization. Biointerphases. 2011 Mar;6(1):8–15.
Yang P, Marinakos SM, Chilkoti A. Spatially addressable chemoselective C-terminal ligation of an intein fusion protein from a complex mixture to a hydrazine-terminated surface. Langmuir : the ACS journal of surfaces and colloids. 2011 Feb;27(4):1463–71.
Amiram M, Quiroz FG, Callahan DJ, Chilkoti A. A highly parallel method for synthesizing DNA repeats enables the discovery of 'smart' protein polymers. Nat Mater. 2011 Feb;10(2):141–8.
Crow MJ, Marinakos SM, Cook JM, Chilkoti A, Wax A. Plasmonic flow cytometry by immunolabeled nanorods. Cytometry A. 2011 Jan;79(1):57–65.
Williams C, Brown XQ, Bartolak-Suki E, Ma H, Chilkoti A, Wong JY. The use of micropatterning to control smooth muscle myosin heavy chain expression and limit the response to transforming growth factor β1 in vascular smooth muscle cells. Biomaterials. 2011 Jan;32(2):410–8.
Chen M, McDaniel JR, Mackay JA, Chilkoti A. NANOSCALE SELF-ASSEMBLY FOR DELIVERY OF THERAPEUTICS AND IMAGING AGENTS. Technology and innovation. 2011 Jan;13(1):5–25.
Nettles DL, Chilkoti A, Setton LA. Applications of elastin-like polypeptides in tissue engineering. Advanced drug delivery reviews. 2010 Dec;62(15):1479–85.
McDaniel JR, Callahan DJ, Chilkoti A. Drug delivery to solid tumors by elastin-like polypeptides. Advanced drug delivery reviews. 2010 Dec;62(15):1456–67.
Chen S-Y, Mock JJ, Hill RT, Chilkoti A, Smith DR, Lazarides AA. Gold nanoparticles on polarizable surfaces as Raman scattering antennas. ACS nano. 2010 Nov;4(11):6535–46.
Mackay JA, Callahan DJ, Fitzgerald KN, Chilkoti A. Quantitative model of the phase behavior of recombinant pH-responsive elastin-like polypeptides. Biomacromolecules. 2010 Nov;11(11):2873–9.
Fluegel S, Fischer K, McDaniel JR, Chilkoti A, Schmidt M. Chain stiffness of elastin-like polypeptides. Biomacromolecules. 2010 Nov;11(11):3216–8.
Hill RT, Mock JJ, Urzhumov Y, Sebba DS, Oldenburg SJ, Chen S-Y, et al. Leveraging nanoscale plasmonic modes to achieve reproducible enhancement of light. Nano letters. 2010 Oct;10(10):4150–4.
Gao W, Liu W, Christensen T, Zalutsky MR, Chilkoti A. In situ growth of a PEG-like polymer from the C terminus of an intein fusion protein improves pharmacokinetics and tumor accumulation. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16432–7.
Hassouneh W, Christensen T, Chilkoti A. Elastin-like polypeptides as a purification tag for recombinant proteins. Current protocols in protein science. 2010 Aug;Chapter 6:6.11.1-6.11.16.
MacEwan SR, Callahan DJ, Chilkoti A. Stimulus-responsive macromolecules and nanoparticles for cancer drug delivery. Nanomedicine (London, England). 2010 Jul;5(5):793–806.
Alang Ahmad S, Hucknall A, Chilkoti A, Leggett GJ. Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes. Langmuir : the ACS journal of surfaces and colloids. 2010 Jun;26(12):9937–42.
Liu W, MacKay JA, Dreher MR, Chen M, McDaniel JR, Simnick AJ, et al. Injectable intratumoral depot of thermally responsive polypeptide-radionuclide conjugates delays tumor progression in a mouse model. J Control Release. 2010 May 21;144(1):2–9.
Jenness NJ, Hill RT, Hucknall A, Chilkoti A, Clark RL. A versatile diffractive maskless lithography for single-shot and serial microfabrication. Optics express. 2010 May;18(11):11754–62.
McDaniel JR, Mackay JA, Quiroz FG, Chilkoti A. Recursive directional ligation by plasmid reconstruction allows rapid and seamless cloning of oligomeric genes. Biomacromolecules. 2010 Apr;11(4):944–52.
Simnick AJ, Valencia CA, Liu R, Chilkoti A. Morphing low-affinity ligands into high-avidity nanoparticles by thermally triggered self-assembly of a genetically encoded polymer. ACS nano. 2010 Apr;4(4):2217–27.
MacEwan SR, Chilkoti A. Elastin-like polypeptides: biomedical applications of tunable biopolymers. Biopolymers. 2010 Jan;94(1):60–77.
Nettles DL, Haider MA, Chilkoti A, Setton LA. Neural network analysis identifies scaffold properties necessary for in vitro chondrogenesis in elastin-like polypeptide biopolymer scaffolds. Tissue engineering Part A. 2010 Jan;16(1):11–20.
Meyer JN, Lord CA, Yang XY, Turner EA, Badireddy AR, Marinakos S, et al. Intracellular uptake and associated toxicity of silver nanoparticles in Caenorhabditis elegans. Aquatic Toxicol. 2010;100:140–50.
Cho Y, Sagle LB, Iimura S, Zhang Y, Kherb J, Chilkoti A, et al. Hydrogen bonding of beta-turn structure is stabilized in D(2)O. Journal of the American Chemical Society. 2009 Oct;131(42):15188–93.
Gao W, Liu W, Mackay JA, Zalutsky MR, Toone EJ, Chilkoti A. In situ growth of a stoichiometric PEG-like conjugate at a protein's N-terminus with significantly improved pharmacokinetics. Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15231–6.
Nettles DL, Chilkoti A, Setton LA. Early metabolite levels predict long-term matrix accumulation for chondrocytes in elastin-like polypeptide biopolymer scaffolds. Tissue engineering Part A. 2009 Aug;15(8):2113–21.
Christensen T, Amiram M, Dagher S, Trabbic-Carlson K, Shamji MF, Setton LA, et al. Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins. Protein science : a publication of the Protein Society. 2009 Jul;18(7):1377–87.
Hucknall A, Rangarajan S, Chilkoti A. In pursuit of zero: Polymer brushes that resist the adsorption of proteins. Advanced Materials. 2009 Jun 19;21(23):2441–6.
Crow M, Marinakos S, Chilkoti A, Wax A. Simultaneous molecular imaging of EGFR and HER2 using hyperspectral darkfield microscopy and immunotargeted nanoparticles. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2009 Jun 12;7192.
Zauscher S, Chilkoti A. Biological applications of polymer brushes. Preface. Biointerphases. 2009 Jun;4(2):FA1–2.
Hucknall A, Simnick AJ, Hill RT, Chilkoti A, Garcia A, Johannes MS, et al. Versatile synthesis and micropatterning of nonfouling polymer brushes on the wafer scale. Biointerphases. 2009 Jun;4(2):FA50–7.
Hucknall A, Kim D-H, Rangarajan S, Hill RT, Reichert WM, Chilkoti A. Simple Fabrication of Antibody Microarrays on Nonfouling Polymer Brushes with Femtomolar Sensitivity for Protein Analytes in Serum and Blood. Advanced materials (Deerfield Beach, Fla). 2009 May;21(19):1968–71.
Nusz GJ, Curry AC, Marinakos SM, Wax A, Chilkoti A. Rational selection of gold nanorod geometry for label-free plasmonic biosensors. ACS nano. 2009 Apr;3(4):795–806.
Chilkoti A. Precision engineered control of biomolecules at interfaces by polymer brushes. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2009 Mar 22;237.
Wu YQ, Mackay JA, Mcdaniel JR, Chilkoti A, Clark RL. Fabrication of Elastin-Like polypeptide Nanoparticles for Drug Delivery by Electrospraying. Biomacromolecules. 2009 Jan;10(1):19–24.
MacKay JA, Chen M, McDaniel JR, Liu W, Simnick AJ, Chilkoti A. Self-assembling chimeric polypeptide–doxorubicin conjugate nanoparticles that abolish tumours after a single injection. Nature Materials. 2009;8:993–9.
Chilkoti A. Designing interfaces for optical biosensors. In: Optics InfoBase Conference Papers. 2009.
MacKay JA, Chen M, McDaniel JR, Liu W, Simnick AJ, Chilkoti A. Self-assembling chimeric polypeptide-doxorubicin conjugate nanoparticles that abolish tumours after a single injection. NATURE MATERIALS. 2009;8(12):993–9.
Chilkoti A. Designing interfaces for optical biosensors. Optics InfoBase Conference Papers. 2009 Jan 1;
Chilkoti A. Patterning materials with biomolecules. 8th World Biomaterials Congress 2008. 2008 Dec 1;4:2249–50.
Kim B, Chilkoti A. Allosteric actuation of inverse phase transition of a stimulus-responsive fusion polypeptide by ligand binding. Journal of the American Chemical Society. 2008 Dec;130(52):17867–73.
Cho YH, Zhang YJ, Christensen T, Sagle LB, Chilkoti A, Cremer PS. Effects of Hofmeister Anions on the Phase Transition Temperature of Elastin-like Polypeptides. Journal Of Physical Chemistry B. 2008 Nov;112(44):13765–71.
Mackay JA, Chilkoti A. Temperature sensitive peptides: engineering hyperthermia-directed therapeutics. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group. 2008 Sep;24(6):483–95.
Xu F, Joon HM, Trabbic-Carlson K, Chilkoti A, Knoll W. Surface plasmon optical study of the interfacial phase transition of elastinlike polypeptide grafted on gold. Biointerphases. 2008 Sep;3(3):66–74.
Valiaev A, Dong WL, Schmidler S, Clark RL, Chilkoti A, Zauscher S. Hydration and conformational mechanics of single, end-tethered elastin-like polypeptides. Journal of the American Chemical Society. 2008 Aug 20;130(33):10939–46.
Chilkoti A. PMSE 446-Architecture of polymer-drug conjugate controls in vivo fate and efficacy. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2008.
Shamji MF, Chen J, Friedman AH, Richardson WJ, Chilkoti A, Setton LA. Synthesis and characterization of a thermally-responsive tumor necrosis factor antagonist. J Control Release. 2008 Aug 7;129(3):179–86.
Mock JJ, Hill RT, Degiron A, Zauscher S, Chilkoti A, Smith DR. Distance-dependent plasmon resonant coupling between a gold nanoparticle and gold film. Nano letters. 2008 Aug;8(8):2245–52.
Nettles DL, Kitaoka K, Hanson NA, Flahiff CM, Mata BA, Hsu EW, et al. In situ crosslinking elastin-like polypeptide gels for application to articular cartilage repair in a goat osteochondral defect model. Tissue engineering Part A. 2008 Jul;14(7):1133–40.
Chilkoti A. PMSE 206-Architecture of polymer-drug conjugate controls in vivo fate and efficacy. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2008.
Chilkoti A. POLY 351-Thermally responsive elastin biopolymers for drug delivery. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2008.
Shamji MF, Whitlatch L, Friedman AH, Richardson WJ, Chilkoti A, Setton LA. An injectable and in situ-gelling biopolymer for sustained drug release following perineural administration. Spine (Phila Pa 1976). 2008 Apr 1;33(7):748–54.
Nusz GJ, Marinakos SM, Curry AC, Dahlin A, Höök F, Wax A, et al. Label-free plasmonic detection of biomolecular binding by a single gold nanorod. Analytical chemistry. 2008 Feb;80(4):984–9.
Skala MC, Marinakos S, Chilkoti A, Izatt JA. Photothermal optical coherence tomography for molecular contrast imaging. Biomedical Optics, BIOMED 2008. 2008 Jan 1;
Lim DW, Nettles DL, Setton LA, Chilkoti A. In situ cross-linking of elastin-like polypeptide block copolymers for tissue repair. Biomacromolecules. 2008 Jan;9(1):222–30.
Chow D, Nunalee ML, Lim DW, Simnick AJ, Chilkoti A. Peptide-based Biopolymers in Biomedicine and Biotechnology. Materials science & engineering R, Reports : a review journal. 2008 Jan;62(4):125–55.
Dreher MR, Simnick AJ, Fischer K, Smith RJ, Patel A, Schmidt M, et al. Temperature triggered self-assembly of polypeptides into multivalent spherical micelles. Journal of the American Chemical Society. 2008 Jan;130(2):687–94.
Lam Y, Valiaev A, Lim DW, Chilkoti A, Alam SM, Zauscher S. Single molecule nanomechanics of HIV-1 envelope glycoproteins and elastin-like polypeptides. Proceedings of the SEM Annual Conference and Exposition on Experimental and Applied Mechanics 2007. 2007 Nov 19;1:624–5.
Shamji MF, Betre H, Kraus VB, Chen J, Chilkoti A, Pichika R, et al. Development and characterization of a fusion protein between thermally responsive elastin-like polypeptide and interleukin-1 receptor antagonist: sustained release of a local antiinflammatory therapeutic. Arthritis Rheum. 2007 Nov;56(11):3650–61.
Chow DC, Chilkoti A. Surface-initiated enzymatic polymerization of DNA. Langmuir : the ACS journal of surfaces and colloids. 2007 Nov;23(23):11712–7.
Garcia A, Hucknall A, Johannes M, Clark R, Chilkoti A, Zauscher S. POLY 627-Fabrication of bioconjugated and hybrid polymeric nanostructures by field-induced scanning probe lithography. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2007 Aug 19;234.
Chilkoti A. Elastin biopolymers for drug delivery. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2007 Aug 19;234.
Roberts SK, Chilkoti A, Setton LA. Multifunctional thermally transitioning oligopeptides prepared by ring-opening metathesis polymerization. Biomacromolecules. 2007 Aug;8(8):2618–21.
Dreher MR, Chilkoti A. Toward a systems engineering approach to cancer drug delivery. Journal of the National Cancer Institute. 2007 Jul;99(13):983–5.
Marinakos SM, Chen S, Chilkoti A. Plasmonic detection of a model analyte in serum by a gold nanorod sensor. Analytical chemistry. 2007 Jul;79(14):5278–83.
Valiaev A, Lim DW, Oas TG, Chilkoti A, Zauscher S. Force-induced prolyl cis-trans isomerization in elastin-like polypeptides. J Am Chem Soc. 2007 May 23;129(20):6491–7.
Dreher MR, Liu W, Michelich CR, Dewhirst MW, Chilkoti A. Thermal cycling enhances the accumulation of a temperature-sensitive biopolymer in solid tumors. Cancer Res. 2007 May 1;67(9):4418–24.
Lim DW, Trabbic-Carlson K, Mackay JA, Chilkoti A. Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides. Biomacromolecules. 2007 May;8(5):1417–24.
Lim DW, Nettles DL, Setton LA, Chilkoti A. Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution. Biomacromolecules. 2007 May;8(5):1463–70.
Curry A, Nusz G, Chilkoti A, Wax A. Analysis of total uncertainty in spectral peak measurements for plasmonic nanoparticle-based biosensors. Applied optics. 2007 Apr;46(10):1931–9.
Lee W-K, Chen S, Chilkoti A, Zauscher S. Fabrication of gold nanowires by electric-field-induced scanning probe lithography and in situ chemical development. Small (Weinheim an der Bergstrasse, Germany). 2007 Feb;3(2):249–54.
Kim DH, Smith JT, Chilkoti A, Reichert WM. The effect of covalently immobilized rhIL-1ra-ELP fusion protein on the inflammatory profile of LPS-stimulated human monocytes. Biomaterials. 2007;28(23):3369–77.
Simnick AJ, Lim DW, Chow D, Chilkoti A. Biomedical and biotechnological applications of Elastin-like polypeptides. Polymer Reviews. 2007 Jan 1;47(1):121–54.
Dhawan A, Gerhold M, Marinakos S, Leonard D, Wang HN, Chilkoti A, et al. Fabrication of fiber-optic sensors based on plasmon resonances of metallic nanostructures. ECS Transactions. 2007 Jan 1;11(14):41–55.
Valiaev A, Abu-Lail NI, Lim DW, Chilkoti A, Zauscher S. Microcantilever sensing and actuation with end-grafted stimulus-responsive elastin-like polypeptides. Langmuir : the ACS journal of surfaces and colloids. 2007 Jan;23(1):339–44.
Christensen1 T, a, Trabbic-Carlson1 K, Liua W, Chilkoti A. Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling. Analytical Biochemistry. 2007 Jan;Volume 360, Issue 1:166–8.
Chilkoti A, Christensen T, MacKay JA. Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology. Current opinion in chemical biology. 2006 Dec;10(6):652–7.
Liu W, Dreher MR, Furgeson DY, Peixoto KV, Yuan H, Zalutsky MR, et al. Tumor accumulation, degradation and pharmacokinetics of elastin-like polypeptides in nude mice. J Control Release. 2006 Nov 28;116(2):170–8.
Liu WE, Dreher MR, Furgeson DY, Peixoto KV, Yuan H, Zalutsky MR, et al. Tumor accumulation, degradation and pharmacokinetics of elastin-like polypeptides in nude mice. Journal Of Controlled Release. 2006 Nov;116(2):170–8.
Betre H, Liu W, Zalutsky MR, Chilkoti A, Kraus VB, Setton LA. A thermally responsive biopolymer for intra-articular drug delivery. J Control Release. 2006 Oct 10;115(2):175–82.
Ge X, Trabbic-Carlson K, Chilkoti A, Filipe CDM. Purification of an elastin-like fusion protein by microfiltration. Biotechnology and bioengineering. 2006 Oct;95(3):424–32.
Liu W, Dreher MR, Chow DC, Zalutsky MR, Chilkoti A. Tracking the in vivo fate of recombinant polypeptides by isotopic labeling. J Control Release. 2006 Aug 28;114(2):184–92.
Chow DC, Dreher MR, Trabbic-Carlson K, Chilkoti A. Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli. Biotechnology progress. 2006 May;22(3):638–46.
Pyhtila JW, Ma H, Simnick AJ, Chilkoti A, Wax A. Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry. Journal of biomedical optics. 2006 May;11(3):34022.
Ma H, Li D, Sheng X, Zhao B, Chilkoti A. Protein-resistant polymer coatings on silicon oxide by surface-initiated atom transfer radical polymerization. Langmuir : the ACS journal of surfaces and colloids. 2006 Apr;22(8):3751–6.
Lee W-K, Chen S, Chilkoti A, Zauscher S. Fabrication of bioconjugated polymeric nanostructures and metal nanowires by electric field-induced scanning probe lithography. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2006 Mar 26;231.
Chilkoti A. Bionanofabrication with polymers and enzymes. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2006 Mar 26;231.
Abu-Lail N, Kaholek M, Valiaev A, Lim D-W, Chilkoti A, LaMattina B, et al. Micro-cantilevers decorated with tethered stimulus-responsive polymer brushes and polypeptides for actuation and sensing. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2006 Mar 26;231.
Ma H, Wells M, Beebe TP, Chilkoti A. Surface-initiated atom transfer radical polymerization of oligo(ethylene glycol) methyl methacrylate from a mixed self-assembled monolayer on gold. Advanced Functional Materials. 2006 Mar 20;16(5):640–8.
Ong SR, Trabbic-Carlson KA, Nettles DL, Lim DW, Chilkoti A, Setton LA. Epitope tagging for tracking elastin-like polypeptides. Biomaterials. 2006 Mar;27(9):1930–5.
Dreher MR, Liu W, Michelich CR, Dewhirst MW, Yuan F, Chilkoti A. Tumor vascular permeability, accumulation, and penetration of macromolecular drug carriers. J Natl Cancer Inst. 2006 Mar 1;98(5):335–44.
Hyun J, Chen J, Setton LA, Chilkoti A. Patterning cells in highly deformable microstructures: effect of plastic deformation of substrate on cellular phenotype and gene expression. Biomaterials. 2006 Mar;27(8):1444–51.
Ma H, Textor M, Clark RL, Chilkoti A. Monitoring kinetics of surface initiated atom transfer radical polymerization by quartz crystal microbalance with dissipation. Biointerphases. 2006 Mar;1(1):35.
Curry A, Nusz G, Chilkoti A, Wax A. Measurement system for the high-throughput characterization of metal nanoparticles for biosensors. Optics InfoBase Conference Papers. 2006 Jan 1;
Zhang Y, Trabbic-Carlson K, Albertorio F, Chilkoti A, Cremer PS. Aqueous two-phase system formation kinetics for elastin-like polypeptides of varying chain length. Biomacromolecules. 2006;7(7):2192–9.
Furgeson DY, Dreher MR, Chilkoti A. Structural optimization of a "smart" doxorubicin-polypeptide conjugate for thermally targeted delivery to solid tumors. Journal of controlled release : official journal of the Controlled Release Society. 2006 Jan;110(2):362–9.
Betre H, Ong SR, Guilak F, Chilkoti A, Fermor B, Setton LA. Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide. Biomaterials. 2006 Jan;27(1):91–9.
Buchheit TE, Minor AM, Spolenak R, Takashima K. Materials Research Society Symposium Proceedings: Preface. Materials Research Society Symposium Proceedings. 2005 Dec 1;875.
Haider MA, Nettles DL, Trabbic-Carlson K, Chilkoti A, Setton LA. Predictive modeling of polypeptide hydrogel mechanical properties for cartilage repair using artificial neural networks. Proceedings of the 2005 Summer Bioengineering Conference. 2005 Dec 1;2005:633–4.
McHale MK, Setton LA, Chilkoti A. Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair. Tissue engineering. 2005 Nov;11(11–12):1768–79.
Chow DC, Lee W-K, Zauscher S, Chilkoti A. Enzymatic fabrication of DNA nanostructures: extension of a self-assembled oligonucleotide monolayer on gold arrays. Journal of the American Chemical Society. 2005 Oct;127(41):14122–3.
Chilkoti A. Targeted drug delivery to solid tumors by thermally responsive polypeptides. European Cells and Materials. 2005 Sep 1;10(SUPPL.5):11.
Valiaev A, Abu-Lail NI, Lim DW, Chilkoti A, Zauscher S. Stimulus-responsive elastin-like polypetides as coatings for microcantilevers: Applications for sensing and actuation. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2005 Aug 28;230:U4317–8.
Chow DC, Chilkoti A, Lee WK, Zauscher S. Rapid fabrication of DNA nanostructures by enzymatic reactions. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2005 Aug 28;230:U1069–U1069.
Chilkoti A. Passive and active surfaces to control protein and cell interactions. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2005 Aug 28;230:U4150–1.
Ge X, Yang DSC, Trabbic-Carlson K, Kim B, Chilkoti A, Filipe CDM. Self-cleavable stimulus responsive tags for protein purification without chromatography. Journal of the American Chemical Society. 2005 Aug;127(32):11228–9.
Curry A, Nusz G, Chilkoti A, Wax A. Substrate effect on refractive index dependence of plasmon resonance for individual silver nanoparticles observed using darkfield microspectroscopy. Optics express. 2005 Apr;13(7):2668–77.
Chilkoti A. Nano- to micro-scale structures of polymers that repel and attract proteins. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2005 Mar 13;229:U1158–U1158.
Chilkoti A, Hubbell JA. Biointerface Science. MRS Bulletin. 2005 Mar;30(3):175–9.
Ma H, Hyun J, Zhang Z, Jr BTP, Chilkoti A. Fabrication of biofunctionalized quasi-three-dimensional microstructures of a nonfouling comb polymer using soft lithography. Adv Funct Mater (Germany). 2005;15(4):529–40.
Dagher S, Rose D, Chilkoti A. Novel technology for cost-effective expression and purification of recombinant proteins and peptides. In: BIOPOLYMERS. JOHN WILEY & SONS INC; 2005. p. 538–9.
Valiaev A, Lim DW, Oas T, Clark RL, Chilkoti A, Zauscher S. Force-induced proline cis-trans isomerization in elastin-like polypeptides. BIOPHYSICAL JOURNAL. 2005 Jan 1;88(1):168A-168A.
Curry A, Nusz G, Chilkoti A, Wax A. Measurement system for the high-throughput characterization of metal nanoparticles for biosensors. Optics InfoBase Conference Papers. 2005 Jan 1;
Zhang Z, Ma H, Hausner DB, Chilkoti A, Beebe Jr TP. Pretreatment of amphiphilic comb polymer surfaces dramatically affects protein adsorption. Biomacromolecules. 2005;6(6):3388–96.
Chow DC, Johannes MS, Lee WK, Clark RL, Zauscher S, Chilkoti A. Nanofabrication with biomolecules. Materials Today. 2005 Jan 1;8(12 SUPPL. 1):30–9.
Dagher S, Rose D, Chilkoti A. Novel technology for cost-effective expression and purification of recombinant proteins and peptides. In: BIOPOLYMERS. JOHN WILEY & SONS INC; 2005. p. 530–1.
Valiaev A, Dong WL, Chilkoti A, Schmidler S, Zauscher S. Polypeptide-solvent interactions measured by single molecule force spectroscopy. Materials Research Society Symposium Proceedings. 2005;898:92–7.
Curry A, Nusz G, Chilkoti A, Wax A. Measurement system for the high-throughput characterization of metal nanoparticles for biosensors. 2005 Conference on Lasers and Electro-Optics, CLEO. 2005 Jan 1;3:2160–2.
Wong JY, Plant AL, Schmidt CE, Shea L, Coury AJ, Chen CS, et al. Materials Research Society Symposium Proceedings: Preface. In: Materials Research Society Symposium Proceedings. 2004.
Trabbic-Carlson K, Liu L, Kim B, Chilkoti A. Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion. Protein science : a publication of the Protein Society. 2004 Dec;13(12):3274–84.
Nath N, Hyun J, Ma H, Chilkoti A. Surface engineering strategies for control of protein and cell interactions. Surface Science. 2004 Oct 10;570(1–2):98–110.
Dreher MR, Elas M, Ichikawa K, Barth ED, Chilkoti A, Rosen GM, et al. Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry. Med Phys. 2004 Oct;31(10):2755–62.
Nath N, Chilkoti A. Label-free biosensing by surface plasmon resonance of nanoparticles on glass: optimization of nanoparticle size. Analytical chemistry. 2004 Sep;76(18):5370–8.
Valiaev A, Lim DW, Clark R, Chilkoti A, Zauscher S. Mechanics of stimulus-responsive elastin-like polypeptides studied by force spectroscopy. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2004. p. U485–U485.
Nath N, Chilkoti A. Label free colorimetric biosensing using nanoparticles. Journal of fluorescence. 2004 Jul;14(4):377–89.
Hyun J, Lee W-K, Nath N, Chilkoti A, Zauscher S. Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches". Journal of the American Chemical Society. 2004 Jun;126(23):7330–5.
Meyer DE, Chilkoti A. Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides. Biomacromolecules. 2004 May;5(3):846–51.
Hyun J, Kim J, Craig SL, Chilkoti A. Enzymatic nanolithography of a self-assembled oligonucleotide monolayer on gold. Journal of the American Chemical Society. 2004 Apr;126(15):4770–1.
Kaholek M, Hyun J, Lee WK, Chilkoti A, Zauscher S. Fabrication of "smart" biomolecular and polymeric nanostructures using molecular recognition, surface-initiated nanopolymerization and scanning probe lithography. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2004. p. U861–U861.
Ma H, Hyun J, Stiller P, Chilkoti A. "Non-Fouling" Oligo(ethylene glycol)-Functionalized Polymer Brushes Synthesized by Surface-Initiated Atom Transfer Radical Polymerization. Advanced Materials. 2004 Feb 17;16(4):338–41.
Prohaska S, Dreher M, Dewhirst MW, Chilkoti A, Pries AR, Westerhoff M, et al. 3-D reconstruction of tumor vascular networks. In: JOURNAL OF VASCULAR RESEARCH. KARGER; 2004. p. 463–463.
Trabbic-Carlson K, Meyer DE, Liu L, Piervincenzi R, Nath N, LaBean T, et al. Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity? Protein engineering, design & selection : PEDS. 2004 Jan;17(1):57–66.
Piervincenzi RT, Chilkoti A. Effect of genetic circular permutation near the active site on the activity and stability of an enzyme inhibitor. Biomolecular engineering. 2004 Jan;21(1):33–42.
Valiaev A, Clark RL, Chilkoti A, Zauscher S. Conformational Mechanics of Stimulus-Responsive Polypeptides. Proceedings of SPIE - The International Society for Optical Engineering. 2003 Nov 27;5053:31–40.
Blomfield CJ, Roberts AJ, Hutton SJ, Ma H, Hyun J, Chilkoti A. X-ray photoelectron spectroscopy as a tool for bio-surface analysis. European Cells and Materials. 2003 Sep 1;6(SUPPL. 1):60.
Ma H, Hyun J, Nath N, Chilkoti A. Surface engineering strategies for control of protein and cell interactions. European Cells and Materials. 2003 Sep 1;6(SUPPL. 1):16–7.
Zauscher S, Chilkoti A, Hyun J, Lee WK. Fabrication of "smart" protein nanostructures using molecular recognition and dip-pen nano-lithography. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2003. p. U485–U485.
Nath N, Chilkoti A, Zauscher S, Hyun JH, Lee WK. Non-covalent, environmentally modulated molecular recognition between ELP biopolymers: A convenient route to reversible protein arrays. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2003. p. U410–U410.
Dreher MR, Raucher D, Balu N, Michael Colvin O, Ludeman SM, Chilkoti A. Evaluation of an elastin-like polypeptide-doxorubicin conjugate for cancer therapy. Journal of controlled release : official journal of the Controlled Release Society. 2003 Aug;91(1–2):31–43.
Trabbic-Carlson K, Setton LA, Chilkoti A. Swelling and mechanical behaviors of chemically cross-linked hydrogels of elastin-like polypeptides. Biomacromolecules. 2003 May;4(3):572–80.
Frey W, Meyer DE, Chilkoti A. Thermodynamically reversible addressing of a stimuli responsive fusion protein onto a patterned surface template. Langmuir. 2003 Mar 4;19(5):1641–53.
Chilkoti A, Ma HW, Hyun J, Nath N. Passive and active nonfouling polymer grafts. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2003. p. U654–5.
Chilkoti A. Nanoparticle biosensors. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2003. p. U964–U964.
Zauscher S, Chilkoti A, Ahn SJ, Hyun J, Lee WK. Fabrication of surface confined, stimulus-responsive polymer nanostructures using dip-pen nanolithography. In: ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. AMER CHEMICAL SOC; 2003. p. U655–U655.
Frey W, Meyer DE, Chilkoti A. Dynamic addressing of a surface pattern by a stimuli-responsive fusion protein. Advanced Materials. 2003 Feb 5;15(3):248–51.
Chan BP, Chilkoti A, Reichert WM, Truskey GA. Effect of streptavidin affinity mutants on the integrin-independent adhesion of biotinylated endothelial cells. Biomaterials. 2003 Feb;24(4):559–70.
Nath N, Chilkoti A. Fabrication of a reversible protein array directly from cell lysate using a stimuli-responsive polypeptide. Analytical chemistry. 2003 Feb;75(4):709–15.
Hyun J, Ma H, Zhang Z, Beebe Jr TP, Chilkoti A. Universal route to cell micropatterning using an amphiphilic comb polymer. Advanced Materials. 2003;15(7–8):576–9.
Nath N, Chilkoti A. A colorimetric gold nanoparticle biosensor: Effect of particle size on sensitivity. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2002 Dec 1;1:574–5.
Dreher M, Raucher D, Balu N, Colvin M, Ludeman S, Chilkoti A. Elastin-like polypeptide-doxorubicin conjugates for cancer therapy. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2002 Dec 1;1:524–5.
Betre H, Chilkoti A, Setton LA. A two-step chondrocyte recovery system based on thermally sensitive elastin-like polypeptide scaffolds for cartilage tissue engineering. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 2002 Dec 1;1:829–30.
Chilkoti A, Dreher MR, Meyer DE. Design of thermally responsive, recombinant polypeptide carriers for targeted drug delivery. Advanced drug delivery reviews. 2002 Oct;54(8):1093–111.
Betre H, Setton LA, Meyer DE, Chilkoti A. Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair. Biomacromolecules. 2002 Sep;3(5):910–6.
Chilkoti A, Dreher MR, Meyer DE, Raucher D. Targeted drug delivery by thermally responsive polymers. Advanced drug delivery reviews. 2002 Sep;54(5):613–30.
Chilkoti A, Nath N. A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real-time on a surface. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2002 Aug 18;224:U151–U151.
Hyun J, Ma H, Banerjee P, Cole J, Gonsalves K, Chilkoti A. Micropatterns of a cell-adhesive peptide on an amphiphilic comb polymer film. Langmuir. 2002 Apr 16;18(8):2975–9.
Rama Rao GV, Balamurugan S, Meyer DE, Chilkoti A, López GP. Hybrid bioinorganic smart membranes that incorporate protein-based molecular switches. Langmuir. 2002 Mar 5;18(5):1819–24.
Meyer DE, Chilkoti A. Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system. Biomacromolecules. 2002 Mar;3(2):357–67.
Nath N, Chilkoti A. A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface. Analytical chemistry. 2002 Feb;74(3):504–9.
Nath N, Chilkoti A. An immobilized gold nanoparticle sensor for label free optical detection of biomolecular interactions. Proceedings of SPIE - The International Society for Optical Engineering. 2002 Jan 1;4626:441–8.
Nath N, Chilkoti A. Creating "smart" surfaces using stimuli responsive polymers. Advanced Materials. 2002;14(17):1243–7.
Hyun J, Ahn SJ, Lee WK, Chilkoti A, Zauscher S. Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography. Nano Lett (USA). 2002;2(11):1203–7.
Hyun J, Zhu Y, Liebmann-Vinson A, Beebe TP, Chilkoti A. Microstamping on an activated polymer surface: Patterning biotin and streptavidin onto common polymeric biomaterials. Langmuir. 2001 Oct 2;17(20):6358–67.
Raucher D, Chilkoti A. Enhanced uptake of a thermally responsive polypeptide by tumor cells in response to its hyperthermia-mediated phase transition. Cancer research. 2001 Oct;61(19):7163–70.
Chilkoti A. Biomedical applications of genetically encoded elastin biopolymers. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2001 Aug 1;222:U421–U421.
Nath N, Chilkoti A. Interfacial phase transition of an environmentally responsive elastin biopolymer adsorbed on functionalized gold nanoparticles studied by colloidal surface plasmon resonance. Journal of the American Chemical Society. 2001 Aug;123(34):8197–202.
Hyun J, Chilkoti A. Surface-initiated free radical polymerization of polystyrene micropatterns on a self-assembled monolayer on gold. Macromolecules. 2001 Jul 31;34(16):5644–52.
Meyer DE, Shin BC, Kong GA, Dewhirst MW, Chilkoti A. Drug targeting using thermally responsive polymers and local hyperthermia. J Control Release. 2001 Jul 6;74(1–3):213–24.
Meyer DE, Trabbic-Carlson K, Chilkoti A. Protein purification by fusion with an environmentally responsive elastin-like polypeptide: effect of polypeptide length on the purification of thioredoxin. Biotechnology progress. 2001 Jul;17(4):720–8.
Hyun J, Chilkoti A. Micropatterning biological molecules on a polymer surface using elastomeric microwells. Journal of the American Chemical Society. 2001 Jul;123(28):6943–4.
Chilkoti A, Nath N. Phase transition of environmentally responsive elastin biopolymers adsorbed on gold nanoparticles studied by colloidal surface plasmon resonance. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2001 Apr 1;221:U366–U366.
Meyer DE, Kong GA, Dewhirst MW, Zalutsky MR, Chilkoti A. Targeting a genetically engineered elastin-like polypeptide to solid tumors by local hyperthermia. Cancer Res. 2001 Feb 15;61(4):1548–54.
Belu AM, Yang Z, Aslami R, Chilkoti A. Enhanced TOF-SIMS imaging of a micropatterned protein by stable isotope protein labeling. Analytical chemistry. 2001 Jan;73(2):143–50.
Chilkoti A, Hyun J, Belu A, Yang Z. Microstamping of biological ligands on activated polymer surfaces. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
Hoffman AS, Stayton PS, Bulmus V, Chen G, Chen J, Cheung C, et al. Founder's Award, Society for Biomaterials. Sixth World Biomaterials Congress 2000, Kamuela, HI,May 15-20, 2000. Really smart bioconjugates of smart polymers and receptor proteins. Journal of biomedical materials research. 2000 Dec;52(4):577–86.
Meyer D, Chilkoti A. Elastin-like polypeptides as thermally targeted drug carriers. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
Meyer D, Chilkoti A. Engineered fusion protein of an elastin-like polypeptide and autofluorescent proteins: a genetically encodable nanobiosensor. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
Frey W, Chilkoti A. Ultraflat nanosphere lithography: functionalized nanostructures for biomedical applications. Annals of Biomedical Engineering. 2000 Dec 1;28(SUPPL. 1).
Yang Z, Belu AM, Liebmann-Vinson A, Sugg H, Chilkoti A. Molecular imaging of a micropatterned biological ligand on an activated polymer surface. Langmuir. 2000 Sep 1;16(19):7482–92.
Chilkoti A, Yang ZP, Belu AM. Microstamping of biological ligands on activated polymer surfaces. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2000 Mar 26;219:U573–U573.
Chilkoti A, Meyer DE, Kong GH, Dewhirst MW, Foulon C, Zalutsky MR. Genetically engineered biopolymer conjugate for thermal targeting of anticancer therapeutics. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 2000 Mar 26;219:U453–U453.
Stayton PS, Hoffman AS, Murthy N, Lackey C, Cheung C, Tan P, et al. Molecular engineering of proteins and polymers for targeting and intracellular delivery of therapeutics. Journal of controlled release : official journal of the Controlled Release Society. 2000 Mar;65(1–2):203–20.
Yang Z, Chilkoti A. Microstamping of a Biological Ligand onto an Activated Polymer Surface. Advanced Materials. 2000 Mar;12(6):413–7.
Meyer DE, Kong GA, Dewhirst MW, Foulon C, Zalutsky MR, Chilkoti A. Genetically engineered polypeptide carrier for thermal targeting of anticancer therapeutics. American Chemical Society, Polymer Preprints, Division of Polymer Chemistry. 2000 Mar 1;41(1):1020–1.
Hoffman AS, Stayton PS, Bulmus V, Chen G, Chen J, Cheung C, et al. Really smart bioconjugates of smart polymers and receptor proteins. Journal of Biomedical Materials Research. 2000;52(4):577–86.
Frey W, Woods CK, Chilkoti A. Ultraflat nanosphere lithography: a new method to fabricate flat nanostructures. Adv Mater (Germany). 2000;12(20):1515–9.
Yang Z, Frey W, Oliver T, Chilkoti A. Light-activated affinity micropatterning of proteins on self-assembled monolayers on gold. Langmuir (USA). 2000;16(4):1751–8.
Yang Z, Chilkoti A. Microstamping of a biological ligand onto an activated polymer surface. Advanced Materials. 2000;12(6):413–7.
Frey W, Chilkoti A. Ultraflat nanosphere lithography: functionalized nanostructures for biomedical applications. Annals of Biomedical Engineering. 2000;28(SUPPL 1):94.
Chilkoti A, Hyun J, Belu A, Yang Z. Microstamping of biological ligands on activated polymer surfaces. Annals of Biomedical Engineering. 2000;28(SUPPL 1):92.
Meyer D, Chilkoti A. Engineered fusion protein of an elastin-like polypeptide and autofluorescent proteins: a genetically encodable nanobiosensor. Annals of Biomedical Engineering. 2000;28(SUPPL 1):95.
Wong J, Chilkoti A, Moy VT. Direct force measurements of the streptavidin-biotin interaction. Biomolecular engineering. 1999 Dec;16(1–4):45–55.
Yang ZP, Chilkoti A. Light-activated affinity micropatterning of proteins. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 1999 Dec 1;2:738.
Freitag S, Le Trong I, Klumb LA, Chu V, Chilkoti A, Stayton PS, et al. X-ray crystallographic studies of streptavidin mutants binding to biotin. Biomolecular engineering. 1999 Dec;16(1–4):13–9.
Stayton PS, Freitag S, Klumb LA, Chilkoti A, Chu V, Penzotti JE, et al. Streptavidin-biotin binding energetics. Biomolecular engineering. 1999 Dec;16(1–4):39–44.
Meyer DE, Kong G, Dewhirst M, Chilkoti A. Genetically-engineered polypeptide carrier for thermal targeting of therapeutics. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 1999 Dec 1;2:1289.
Piervincenzi RT, Chilkoti A. Controlling the orientation of immobilized proteins by genetic circular permutation. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 1999 Dec 1;1:93.
Meyer DE, Chilkoti A. Purification of recombinant proteins by fusion with thermally-responsive polypeptides. Nat Biotechnol. 1999 Nov;17(11):1112–5.
Meyer DE, Chilkoti A. Polypeptide fusion tag for thermal purification of recombinant proteins. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. 1999 Mar 21;217:U177–U177.
Freitag S, Le Trong I, Chilkoti A, Klumb LA, Stayton PS, Stenkamp RE. Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex. Journal of molecular biology. 1998 May;279(1):211–21.
Hoffman AS, Stayton PS, Shimoboji T, Chen G, Ding Z, Chilkoti A, et al. Conjugates of Stimuli-Responsive Polymers and Biomolecules: Random and Site-Specific Conjugates of Temperature-Sensitive Polymers and Proteins. Macromolecular Symposia. 1997 Jan 1;118:553–62.
Shimoboji T, Stayton PS, Long C, Chilkoti A, Chen G, Harris JM, et al. Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. 1996 Dec 1;1:897.
Frey W, Schief WR, Pack DW, Chen CT, Chilkoti A, Stayton P, et al. Two-dimensional protein crystallization via metal-ion coordination by naturally occurring surface histidines. Proceedings of the National Academy of Sciences of the United States of America. 1996 May;93(10):4937–41.
Chilkoti A, Boland T, Ratner BD, Stayton PS. The relationship between ligand-binding thermodynamics and protein-ligand interaction forces measured by atomic force microscopy. Biophysical journal. 1995 Nov;69(5):2125–30.
Stayton PS, Shimoboji T, Long C, Chilkoti A, Chen G, Harris JM, et al. Control of protein-ligand recognition using a stimuli-responsive polymer. Nature. 1995 Nov;378(6556):472–4.
Chilkoti A, Schwartz BL, Smith RD, Long CJ, Stayton PS. Engineered chimeric streptavidin tetramers as novel tools for bioseparations and drug delivery. Bio/technology (Nature Publishing Company). 1995 Nov;13(11):1198–204.
Chilkoti A, Schmierer AE, Pérez-Luna VH, Ratner BD. Investigating the relationship between surface chemistry and endothelial cell growth: partial least-squares regression of the static secondary ion mass spectra of oxygen-containing plasma-deposited films. Analytical chemistry. 1995 Sep;67(17):2883–91.
Schwartz BL, Bruce JE, Anderson GA, Hofstadler SA, Rockwood AL, Smith RD, et al. Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization. Journal of the American Society for Mass Spectrometry. 1995 Jun;6(6):459–65.
Chilkoti A, Tan PH, Stayton PS. Site-directed mutagenesis studies of the high-affinity streptavidin-biotin complex: contributions of tryptophan residues 79, 108, and 120. Proceedings of the National Academy of Sciences of the United States of America. 1995 Feb;92(5):1754–8.
Chilkoti A, Stayton PS. Molecular Origins of the Slow Streptavidin—Biotin Dissociation Kinetics. Journal of the American Chemical Society. 1995 Jan 1;117(43):10622–8.
Schwartz BL, Gale DC, Smith RD, Chilkoti A, Stayton PS. Investigation of non‐covalent ligand binding to the intact streptavidin tetramer by electrospray ionization mass spectrometry. Journal of Mass Spectrometry. 1995 Jan 1;30(8):1095–102.
Chilkoti A, Chen G, Stayton PS, Hoffman AS. Site-specific conjugation of a temperature-sensitive polymer to a genetically-engineered protein. Bioconjugate chemistry. 1994 Nov;5(6):504–7.
Chilkoti A, Ratner BD, Briggs D. Static secondary ion mass spectrometric investigation of the surface chemistry of organic plasma-deposited films created from oxygen-containing precursors. 3. Multivariate statistical modeling. Analytical chemistry. 1993 Jul;65(13):1736–45.
Chilkoti A, Lopez GP, Ratner BD, Hearn MJ, Briggs D. Analysis of Polymer Surfaces by SIMS. 16. Investigation of Surface Cross-Linking in Polymer Gels of 2-Hydroxyethyl Methacrylate. Macromolecules. 1993 Jan 1;26(18):4825–32.
Chilkoti A, Ratner BD. X-ray Photoelectron Spectroscopy of Iodine-Doped Nonconjugated Polymers. Chemistry of Materials. 1993 Jan 1;5(6):786–92.
Stayton PS, Chilkoti A, Long CJ, Pettit DK, Tan PHS, Chen G, et al. Engineered proteins for biomaterials. Materials Research Society Symposium Proceedings. 1993 Jan 1;292:77–82.
Ratner BD, Tyler BJ, Chilkoti A. Analysis of biomedical polymer surfaces: polyurethanes and plasma-deposited thin films. Clinical materials. 1993 Jan;13(1–4):71–84.
Chilkoti A, Ratner BD, Briggs D, Reich F. Static secondary ion mass spectrometry of organic plasma‐deposited films created from stable isotope‐labeled precursors. II. Mixtures of acetone and oxygen. Journal of Polymer Science Part A: Polymer Chemistry. 1992 Jan 1;30(7):1261–78.
Ratner BD, Chilkoti A, Castner DG. Contemporary methods for characterizing complex biomaterial surfaces. Clinical Materials. 1992 Jan 1;11(1–4):25–36.
López GP, Chilkoti A, Briggs D, Ratner BD. Substrate temperature effects on film chemistry in plasma deposition of organics. III. Analysis by static secondary ion mass spectrometry. Journal of Polymer Science Part A: Polymer Chemistry. 1992 Jan 1;30(11):2427–41.
Chilkoti A, Ratner BD, Briggs D. Analysis of polymer surfaces by SIMS: Part 15. Oxygen‐functionalized aliphatic homopolymers. Surface and Interface Analysis. 1992 Jan 1;18(8):604–18.
Leggett GJ, Chilkoti A, Ratner BD, Vickerman JC. A tandem SIMS study of poly(vinyl methyl ether). Surface and Interface Analysis. 1992 Jan 1;18(3):210–6.
Ertel SI, Chilkoti A, Horbett TA, Ratner BD. Endothelial cell growth on oxygen-containing films deposited by radio-frequency plasmas: the role of surface carbonyl groups. Journal of biomaterials science Polymer edition. 1991 Dec 1;3(2):163–83.
Leggett GJ, Chilkoti A, Castner DG, Ratner BD, Vickerman JC. Direct emission of molecular fragments during the sputtering of poly(4-hydroxystyrene) and determination of ion structures using tandem secondary ion mass spectrometry. International Journal of Mass Spectrometry and Ion Processes. 1991 Sep 2;108(1):29–39.
Chilkoti A, Ratner BD, Briggs D. Static secondary-ion mass spectrometric investigation of the surface structure of organic plasma-deposited films prepared from stable-isotope-labeled precursors. 1. Carbonyl precursors. Analytical chemistry. 1991 Aug;63(15):1612–20.
Chilkoti A, Castner DG, Ratner BD. Static Secondary Ion Mass Spectrometry and X-Ray Photoelectron Spectroscopy of Deuterium- and Methyl-Substituted Polystyrene. Applied Spectroscopy. 1991 Feb;45(2):209–17.
Chilkoti A, Ratner BD. An X‐ray photoelectron spectroscopic investigation of the selectivity of hydroxyl derivatization reactions. Surface and Interface Analysis. 1991 Jan 1;17(8):567–74.
Ertel SI, Chilkoti A, Horbett TA, Ratner BD. Endothelial cell growth on oxygen-containing films deposited by radio-frequency plasmas: the role of surface carbonyl groups. Journal of biomaterials science Polymer edition. 1991 Jan;3(2):163–83.
Chilkoti A, Ratner BD, Briggs D. Structural elucidation of oxygen-containing plasma deposited films by static secondary ion mass spectrometry. Transactions of the Annual Meeting of the Society for Biomaterials in conjunction with the International Biomaterials Symposium. 1991 Jan 1;14:180.
Chilkoti A, Ratner BD, Briggs D. Plasma-Deposited Polymeric Films Prepared from Carbonyl-Containing Volatile Precursors: XPS Chemical Derivatization and Static SIMS Surface Characterization. Chemistry of Materials. 1991 Jan 1;3(1):51–61.
Chilkoti A, Ratner BD, Briggs D. Static SIMS investigation of the surface structure of plasma deposited films prepared from stable isotope-labeled carbonyl precursors. Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. 1990 Jan 1;62:135–9.
Chilkoti A, Castner DG, Briggs D. Surface characterization of a poly(styrene/p-hydroxystyrene) copolymer series using x-ray photoelectron spectroscopy, static secondary ion mass spectrometry, and chemical derivatization techniques. Journal of Vacuum Science and Technology A: Vacuum, Surfaces and Films. 1990 Jan 1;8(3):2274–82.
Chilkoti A, Ratner BD. Effect of monomer type on the surface chemistry of rf-plasma deposited surfaces prepared for cell culture. Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. 1988 Dec 1;59:258–62.

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